PtdIns-(3,4,5)-P3-biotin sodium
Based on 1 Customer Validation
PtdIns-(3,4,5)-P3-biotin sodium is a Biotin-labeled PtdIns-(3,4,5)-P3 (PI(3,4,5)P3). PI(3,4,5)P3 is a substrate of nuclear phosphatidylinositol 5-phosphatase (PIP5Pase). PI(3,4,5)P3 binds to the N-terminus of RAP1 (repressor activator protein 1) and controls its DNA binding activity.
For research use only. We do not sell to patients.
- Purity : 98.0%
- CAS No.: 1415684-80-8
- Formula: C35H61N3Na4O24P4S
- Molecular Weight:1155.78
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
Transient inactivation of PIP5Pase results in the accumulation of nuclear PI(3,4,5)P3, which binds RAP1 and displaces it from expression sites (ESs), activating transcription of silent ESs and variant surface glycoprotein (VSG) switching[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1415684-80-8
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Appearance Solid
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Molecular Weight 1155.78
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Formula C35H61N3Na4O24P4S
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Color White to off-white
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SMILES
O=P(O)(O[Na])O[C@H]1[C@@H]([C@H]([C@@H]([C@@H]([C@@H]1OP(O)(O[Na])=O)O)OP(OC[C@@H](COC(CCCCCCCNC(CCCC[C@H]2[C@]3([H])[C@](NC(N3)=O)([H])CS2)=O)=O)OC(CCCCCCC)=O)(O[Na])=O)O)OP(O)(O[Na])=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Protocols
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Protocol for Electrophoretic Mobility Shift Assay (EMSA)
Electrophoretic mobility shift assay detects protein-nucleic acid binding by incubating a labeled DNA or RNA probe with purified protein or cell extract, then separating free probe from slower-migrating protein-probe complexes on a native gel. For cancer cells, primary neurons, mouse tumor samples, intestinal organoids, inflammatory macrophages, or drug-treated samples, EMSA can measure transcription-factor DNA binding or RNA-binding protein activity in extracts, but it does not directly measure transcription, protein expression, or chromatin occupancy in intact cells. Specificity is judged by competition with unlabeled wild-type probe, failure of mutated or unrelated competitors to compete, and antibody supershift or disruption when the binding protein identity must be confirmed.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
PtdIns-(3,4,5)-P3-biotin sodium Related Classifications
HY-132276 Related Classifications
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