WS009 A and B, new endothelin receptor antagonists isolated from Streptomyces sp. no. 89009. I. Taxonomy, fermentation, isolation, physico-chemical properties and biological activities

  • J Antibiot (Tokyo). 1992 Jul;45(7):1029-40. doi: 10.7164/antibiotics.45.1029.
S Miyata  1 ,  N Ohhata ,  H Murai ,  Y Masui ,  M Ezaki ,  S Takase ,  M Nishikawa ,  S Kiyoto ,  M Okuhara ,  M Kohsaka
Affiliations
  • 1. Exploratory Research Laboratories, Fujisawa Pharmaceutical Co., Ltd., Ibaraki, Japan.
PMID: 1325433 DOI: 10.7164/antibiotics.45.1029
Abstract

WS009 A and B novel Endothelin Receptor antagonists, have been isolated from the fermentation broth of Streptomyces sp. No. 89009. These antagonists were purified from the culture filtrate followed by Diaion SP-207, DEAE Toyopearl column chromatography and HPLC. WS009 A and B showed selective activity in an Endothelin Receptor binding assay with IC50 of 5.8 x 10(-6) M and 6.7 x 10(-7) M, respectively. On the basis of spectroscopic and chemical evidence, the structures of WS009 A and B have been established as 1 and 3, and are highly hydroxylated benz[a]anthraquinone chromophores.

Products