Cytotoxic withanolides from Tubocapsicum anomalum

  • J Nat Prod. 2007 May;70(5):747-53. doi: 10.1021/np0605541.
Pei-Wen Hsieh  1 ,  Zih-You Huang ,  Jyun-Hong Chen ,  Fang-Rong Chang ,  Ching-Chung Wu ,  Yu-Liang Yang ,  Michael Y Chiang ,  Ming-Hon Yen ,  Shu-Li Chen ,  Hsin-Fu Yen ,  Tilo Lübken ,  Wen-Chun Hung ,  Yang-Chang Wu
Affiliations
  • 1. Graduate Institute of Natural Products, School of Pharmcy, Kaohsiung Medical University, Kaohsiung 807, Taiwan, Republic of China.
PMID: 17417907 DOI: 10.1021/np0605541
Abstract

Fifteen new withanolides (1-8, 11-17) and two known withanolides, withanolide D (9) and 17alpha-hydroxywithanolide D (10), were isolated from the stems, roots, and leaves of Tubocapsicum anomalum using bioassay-directed fractionation. The structures were determined by spectroscopic and chemical methods, and the absolute configurations were established by CD analysis and by the Mosher ester method. The structure of 1 and 3 were further confirmed by X-ray crystallographic analysis. Compounds 1, 4-6, 8-10, and 13 showed significant cytotoxic activity against Hep G2, Hep 3B, A-549, MDA-MB-231, MCF-7, and MRC-5 cell lines.