Determination of amidepin in human plasma by reversed-phase high-performance liquid chromatography with fluorescence detection

  • J Chromatogr. 1991 Apr 19;565(1-2):477-81. doi: 10.1016/0378-4347(91)80413-7.
P Stehlík  1 ,  H Houbová
Affiliations
  • 1. Research Institute for Pharmacy and Biochemistry, Prague, Czechoslovakia.
PMID: 1874897 DOI: 10.1016/0378-4347(91)80413-7
Abstract

An isocratic reversed-phase high-performance liquid chromatographic method for the determination of amidepin has been developed. The method is based on the extraction of alkaline plasma with diethyl ether-dichloromethane, and the injection into the Supelcosil LC-18 column of the evaporated and reconstituted organic phase. After separation, detection is carried out by a fluorescence detector (excitation at 195 nm with no filter). The limit of detection is 10 ng/ml of plasma. The mean coefficient of variation is 12%. The plasma levels after oral administration and after intravenous administration are shown.

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