Genetic Incorporation of ε-N-2-Hydroxyisobutyryl-lysine into Recombinant Histones

  • ACS Chem Biol. 2015 Jul 17;10(7):1599-603. doi: 10.1021/cb501055h.
Han Xiao  1 ,  Weimin Xuan  1 ,  Sida Shao  1 ,  Tao Liu  1 ,  Peter G Schultz  1
Affiliations
  • 1. Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, United States.
PMID: 25909834 DOI: 10.1021/cb501055h
Abstract

Here, we report the evolution of an orthogonal amber suppressor pyrrolysyl-tRNA synthetase (PylRS)/tRNACUA(Pyl) pair that genetically encodes the post-translationally modified amino acid, ε-N-2-hydroxyisobutyryl-lysine (HibK), in bacteria and mammalian cells. HibK is a new type of histone MARK that is widely distributed in histone proteins. The ability to site-specifically incorporate HibK into proteins provides a useful tool to probe the biological function of this newly identified post-translational modification.

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