13,14-seco-Withanolides from Physalis minima with Potential Anti-inflammatory Activity

  • Chem Biodivers. 2016 Jul;13(7):884-90. doi: 10.1002/cbdv.201500282.
Ru Lin  1 ,  Yu-Zhou Guan  1 ,  Rui-Jun Li  1 ,  Xiao-Ming Xu  1 ,  Jian-Guang Luo  2 ,  Ling-Yi Kong  3
Affiliations
  • 1. State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing, 210009, P. R. China.
  • 2. State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing, 210009, P. R. China. [email protected].
  • 3. State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing, 210009, P. R. China. [email protected].
PMID: 27258922 DOI: 10.1002/cbdv.201500282
Abstract

Four new 13,14-seco-withanolides, minisecolides A - D (1 - 4), together with three known analogues 5 - 7, were isolated from the whole Plants of Physalis minima. The structures of new compounds were determined on the basis of spectroscopic analysis, including (1) H-, (13) C-NMR, 2D-NMR (HMBC, HSQC, ROESY), and HR-ESI-MS. Evaluation of all isolates for their inhibitory effects on nitric oxide (NO) production was conducted on lipopolysaccaride-activated RAW264.7 macrophages. Compounds 2, 3, 5, and 6 showed inhibitory activities, especially for compound 5 with IC50 value of 3.87 μm.

Keywords
13,14-seco-Withaphysalin; Nitric oxide; Physalis minima; Solanaceae; Withanolides.
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