Synthesis of peptide fragments related to eglin c and examination of their inhibitory effect on human leukocyte elastase, cathepsin G and alpha-chymotrypsin

  • Biochem Biophys Res Commun. 1989 May 30;161(1):272-5. doi: 10.1016/0006-291x(89)91591-x.
Y Okada  1 ,  S Tsuboi ,  Y Tsuda ,  K Nakabayashi ,  Y Nagamatsu ,  J Yamamoto
Affiliations
  • 1. Faculty of Pharmaceutical Sciences, Kobe-Gakuin University, Japan.
PMID: 2730658 DOI: 10.1016/0006-291x(89)91591-x
Abstract

Various kinds of peptide fragments related to eglin c were prepared by the conventional solution method and their inhibitory effects on human leukocyte Elastase, Cathepsin G and alpha-chymotrypsin were examined. Peptide (31-40) inhibited Cathepsin G (Ki = 2.3 x 10(-4) M), peptide (41-49) potently inhibited Cathepsin G and alpha-chymotrypsin (Ki = 4.2 x 10(-5) M and 2.0 x 10(-5) M, respectively), and peptide (60-63) inhibited leukocyte Elastase (Ki = 1.6 x 10(-4) M), whereas, peptide (31-35) weakly inhibited both Elastase and Cathepsin G (Ki = 2.1 x 10(-3) M and 7.3 x 10(-4) M, respectively).

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