Real-time detection and imaging of copper(ii) in cellular mitochondria

  • Org Biomol Chem. 2017 Jan 18;15(3):598-604. doi: 10.1039/c6ob02384c.
Hong Li  1 ,  Ruilong Zhang  1 ,  Chunxia Li  2 ,  Bei Huang  2 ,  Tingting Yu  1 ,  Xiaodan Huang  1 ,  Xuanjun Zhang  3 ,  Fei Li  1 ,  Hongping Zhou  1 ,  Yupeng Tian  1
Affiliations
  • 1. College of Chemistry and Chemical Engineering, Anhui University and Key Laboratory of Functional Inorganic Materials Chemistry of Anhui Province, 230601, Hefei, P.R. China. [email protected].
  • 2. Centre of Stem cell Research and Transformation Medicine, Anhui University, Hefei 230601, P. R. China.
  • 3. Faculty of Health Science, University of Macau, Taipa, Macau SAR, P.R. China.
PMID: 27929196 DOI: 10.1039/c6ob02384c
Abstract

A triphenylamine-pyridine derivative, viz., a fluorescence probe was designed and synthesized. The probe displayed an "on-off-on" fluorescence switch, upon the sequential addition of Cu2+ and ethylenediaminetetraacetic acid (EDTA). The detection mechanism was confirmed by using UV-vis, fluorescence and 1H NMR titration spectra. Interestingly, the probe can accumulate in mitochondria, and thus detect Cu2+ in real-time there, which indicated that probe L can be used to monitor intracellular mitochondrial Cu2+ levels. Further understanding of the biological functions of Cu2+ in mitochondria has important implications for treating Cancer and Other Diseases.

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