Oenothera rosea L´Hér. ex Ait attenuates acute colonic inflammation in TNBS-induced colitis model in rats: in vivo and in silico myeloperoxidase role

  • Biomed Pharmacother. 2018 Dec:108:852-864. doi: 10.1016/j.biopha.2018.09.081.
Natalia Calva-Candelaria  1 ,  María Estela Meléndez-Camargo  1 ,  Hortensia Montellano-Rosales  2 ,  Alan R Estrada-Pérez  3 ,  Martha C Rosales-Hernández  3 ,  M Jonathan Fragoso-Vázquez  4 ,  Marlet Martínez-Archundia  3 ,  José Correa-Basurto  3 ,  Yazmín K Márquez-Flores  5
Affiliations
  • 1. Departamento de Farmacia, Escuela Nacional de Ciencias Biológicas, Campus Zacatenco, Instituto Politécnico Nacional, Av. Wilfrido Massieu s/n Col. Zacatenco, C.P. 07738, Ciudad de México, Mexico.
  • 2. Departamento de Morfología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, Col. Santo Tomás, C.P. 11340, Ciudad de México, Mexico.
  • 3. Laboratorio de Desarrollo de Nuevos Fármacos y Productos Biotecnológicos, Laboratorio de Biofísica y Catálisis, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón s/n, Col. Santo Tomas, C.P. 11340, Ciudad de México, Mexico.
  • 4. Departamento de Química Orgánica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, Col. Santo Tomás, C.P. 11340, Ciudad de México, Mexico.
  • 5. Departamento de Farmacia, Escuela Nacional de Ciencias Biológicas, Campus Zacatenco, Instituto Politécnico Nacional, Av. Wilfrido Massieu s/n Col. Zacatenco, C.P. 07738, Ciudad de México, Mexico; Universidad Tecnológica de México, UNITEC MÉXICO, Campus Marina, Av. Marina Nacional 162 Col. Anáhuac Sección I, C.P. 11320, Miguel Hidalgo, Ciudad de México, Mexico. Electronic address: [email protected].
PMID: 30372897 DOI: 10.1016/j.biopha.2018.09.081
Abstract

Oenothera rosea L´Hér. ex Ait is a species traditionally used in the treatment of inflammation, headache, stomach pain, infections, among Others. The aim of this study was evaluating the acute anti-inflammatory activity of the aqueous extract of O. rosea by 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis. Rats were randomized into six groups: (I) Sham; (II) EtOH; (III) TNBS; and (IV-VI) 250, 500 and 750 mg/Kg, respectively. The colonic injury was induced (groups III-VI) by intrarectal instillation of 0.25 mL of TNBS (10 mg) in 50% ethanol. Groups I and II received an enema (0.25 mL) of physiological saline solution or 50% ethanol, respectively. Treatments were administered by oral gavage 48, 24 and 1 h prior, and 24 h after the induction. The inflammatory response was assessed considering the macroscopic and microscopic damage, the serum nitric oxide (NO), the colonic IL-1β levels, and the myeloperoxidase (MPO) activity. Moreover, we performed an LC-MS-based metabolite profiling, and a docking on the MPO. Doses of 500 and 750 mg/Kg showed a protective effect in the TNBS-induced colonic damage. This activity was related to the downregulation of evaluated parameters. Also, considering previous reports, 29 metabolites of 91 detected were selected for the docking, of which Isolimonic acid (29) and Kaempferol 3-(2'',4''-diacetylrhamnoside) (10) showed the highest affinity to MPO. The aqueous extract of O. rosea protected the TNBS-induced colonic damage in rats, an effect that could be associated with the presence of polyphenolic compounds, Alkaloids, and terpenes; as well as their ability to down-regulate MPO activity.

Keywords
Anti-inflammatory; Docking; Inflammatory bowel disease; Myeloperoxidase; Oenothera rosea; TNBS.
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