Reflexin A, a new indole alkaloid from Rauvolfia reflexa induces apoptosis against colon cancer cells

  • J Asian Nat Prod Res. 2020 May;22(5):474-488. doi: 10.1080/10286020.2019.1588888.
Mehran Fadaeinasab  1 ,  Hamed Karimian  2 ,  Hanita Omar  3 ,  Hairin Taha  4 ,  Arash Khorasani  5 ,  Behrooz Banisalam  5 ,  Kamal Aziz Ketuly  6 ,  Zanariah Abdullah  1
Affiliations
  • 1. Department of Chemistry, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia.
  • 2. School of Medicine, Taylor's University, Subang Jaya Selangor 47500, Malaysia.
  • 3. Centre for Foundation Studies in Science, Division of Chemistry, University of Malaya, Kuala Lumpur 50603, Malaysia.
  • 4. Institute of Energy Infrastructure, Universiti Tenaga Nasional, Kajang 43000, Malaysia.
  • 5. Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia.
  • 6. Department of Medical Chemistry, College of Medicine, University of Duhok, Duhok 78, Kurdistan Region, Iraq.
PMID: 30945944 DOI: 10.1080/10286020.2019.1588888
Abstract

One new indole alkaloid, reflexin A (1), and two known indoles, macusine B (2) and vinorine (3), were isolated from the bark of Rauvolfia reflexa. Their structures were elucidated by 1D and 2D NMR, UV, IR, and MS spectroscopic analyses. Compound 1 displayed Anticancer activity against HCT-116 colon Cancer cells with an IC50 value of 30.24 ± 0.75 µM. The results implied that the newly isolated 1 induced Apoptosis in HCT-116 cells, suggesting its possible role as an Anticancer agent. In vivo acute toxicity study was performed on compound 1 to evaluate its safety profile.

Keywords
HCT-116 cells; Rauvolfia reflexa; acute toxicity; apoptosis; indole alkaloids; reflexin A.
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