Pinoresinol-4-O-β-D-glucopyranoside: a lignan from prunes (Prunus domestica) attenuates oxidative stress, hyperglycaemia and hepatic toxicity in vitro and in vivo

  • J Pharm Pharmacol. 2020 Dec;72(12):1830-1839. doi: 10.1111/jphp.13358.
Fadia S Youssef  1 ,  Mohamed L Ashour  1  2 ,  Hesham A El-Beshbishy  3  4 ,  Alaaeldin Ahmed Hamza  5 ,  Abdel Nasser B Singab  1 ,  Michael Wink  6
Affiliations
  • 1. Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
  • 2. Batterjee Medical College, Jeddah, Saudi Arabia.
  • 3. Medical Laboratory Sciences Department, Fakeeh College for Medical Sciences, Jeddah, Saudi Arabia.
  • 4. Biochemistry Department, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt.
  • 5. Hormone Evaluation Department, National Organization for Drug Control and Research (NODCAR), Giza, Egypt.
  • 6. Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg, Germany.
PMID: 32856312 DOI: 10.1111/jphp.13358
Abstract

Objectives: This study aimed to explore the pharmacological properties of pinoresinol-4-O-β-D-glucopyranoside (PG), isolated from prunes.

Methods: In-vitro antioxidant activity was assessed using ferric reducing antioxidant power (FRAP) and 2,2'-azino-bis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt (ABTS) assays. In-vivo hepatoprotective activity was evaluated using CCl4 -induced hepatotoxicity mouse model. The antihyperglycaemic activity was determined in vitro using α-glucosidase and α-amylase inhibiting activity and in vivo using streptozotocin-treated model. Molecular modelling was done on α-amylase, α-glucosidase, Aldose Reductase and Peroxisome Proliferator-activated Receptor gamma.

Key findings: Pinoresinol-4-O-β-D-glucopyranoside showed promising antioxidant activity in FRAP and ABTS assays with total antioxidant capacity equal 418.47 and 1091.3 µmol/g in terms of ascorbic acid, respectively. PG (50 mg/kg b.w.) exhibited a hepatoprotective activity in vivo as it lowered AST and ALT levels. PG showed a potent in-vitro antihyperglycaemic activity as it inhibited α-glucosidase with an IC50 value of 48.13 μg/ml. PG caused a prominent decline in serum glucose level by 37.83% in streptozotocin-treated mice with promising elevation in Insulin level of 25.37%. Oxidative stress markers were reduced by PG, and it showed a high fitting on α-amylase and α-glucosidase active sites.

Conclusions: Pinoresinol-4-O-β-D-glucopyranoside is a natural entity combating oxidative stress, hepatic damage and diabetes.

Keywords
Prunus domestica; antihyperglycaemic activity; hepatoprotective; molecular modelling; pinoresinol-4-O-β-D-glucopyranoside; streptozotocin.
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