Optimization of Analytical Procedure for In-hospital Rapid Quantification of Serum Level of Favipiravir in the Pharmacological Treatment of COVID-19

  • Anal Sci. 2021 Sep 10;37(9):1301-1304. doi: 10.2116/analsci.21N004.
Yukiko Moriiwa  1 ,  Go Morikawa  2 ,  Katsuko Okazawa  2 ,  Akio Yanagida  1
Affiliations
  • 1. Department of Biomedical Analysis, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences.
  • 2. Department of Pharmacy, Hokushin General Hospital.
PMID: 33612558 DOI: 10.2116/analsci.21N004
Abstract

An in-hospital rapid method for quantifying the serum level of favipiravir (FPV) in the pharmacological treatment of COVID-19 was developed by an appropriate combination of a solid-phase extraction treatment and a reversed-phase HPLC/UV detection system. The quantification method was well-validated and applied to measuring the serum FPV level in a clinical practice at a general hospital that accepts COVID-19 patients. Furthermore, an analysis of data from our preliminary interaction analysis revealed, for the first time, that FPV selectively forms complexes with ferric (Fe3+) and cupric (Cu2+) ions.

Keywords
COVID-19; HPLC; In-hospital quantification; favipiravir; ferric ion; serum level; solid-phase extraction.
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