Development of sensitive and rapid immunoassays for Moniliformin (MON) detection based on nanomaterials labeled monoclonal antibodies

  • Food Chem. 2025 Apr 30:472:142911. doi: 10.1016/j.foodchem.2025.142911.
Lakshani Madushika  1 ,  Yongming Huang  1 ,  Menghan Sun  1 ,  Yuxuan Liu  1 ,  Linfang Lu  1 ,  Xiaoli Li  1 ,  Na Li  1 ,  Kang Jiang  1 ,  Sumei Ling  1 ,  Shihua Wang  2
Affiliations
  • 1. The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, Fujian Key Laboratory of Pathogenic Fungi and Mycotoxins, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
  • 2. The Ministry of Education Key Laboratory of Biopesticide and Chemical Biology, Fujian Key Laboratory of Pathogenic Fungi and Mycotoxins, School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. Electronic address: [email protected].
PMID: 39842210 DOI: 10.1016/j.foodchem.2025.142911
Abstract

Moniliformin (MON) is a toxic secondary metabolite from Fusarium species. The natural contamination of MON in cereals and cereal by-products, poses a risk of exposure to MON. However, so far, no Immunoassay method has been reported to detect MON in field samples. In this study, anti-MON monoclonal antibody (mAb) with high affinity (1 × 108) and isotype of IgG1 was subjected to establish indirect competitive ELISA (ic-ELISA) and immunochromatographic test strips (ICTS). The optimized ic-ELISA resulted in the lowest detection limit (LOD) of 1.55 μg/mL. The LOD value for gold nanoparticles (AuNP) -based test strip was 0.83 μg/mL. The multi branched gold nano-flower particles (AuNFs) reported higher sensitivity with LOD of 0.38 μg/mL. Moreover, the developed test strips exhibited a high specificity without cross-reactivity to related competitive toxins. In conclusion, Immunoassay methods were successfully developed to detect MON in field samples with a short detection time to ensure food security.

Keywords
ELISA; Immunochromatographic test strip; Moniliformin; Monoclonal antibody.
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