Determination of trimipramine and its demethylated and hydroxylated metabolites in plasma by gas chromatography-mass spectrometry

  • J Chromatogr. 1994 Jan 14;652(1):97-103. doi: 10.1016/0378-4347(93)e0366-x.
C B Eap  1 ,  L Koeb ,  P Baumann
Affiliations
  • 1. Unité de Biochimie et Psychopharmacologie Clinique, Départment Universitaire de Psychiatrie Adulte (DUPA), Prilly-Lausanne, Switzerland.
PMID: 8014234 DOI: 10.1016/0378-4347(93)e0366-x
Abstract

A gas chromatographic-mass spectrometric (GC-MS) method has been developed, for the determination of trimipramine (TRI), desmethyltrimipramine (DTRI), didesmethyltrimipramine (DDTRI), 2-hydroxytrimipramine (2-OH-TRI) and 2-hydroxydesmethyltrimipramine (2-OH-DTRI). The method includes two derivatization steps with trifluoroacetic acid anhydride and N-methyl-N-(tert.-butyldimethyl silyl)trifluoroacetamide and the use of an SE-54 capillary silica column. The limits of quantitation were found to be 2 ng/ml for DTRI and 4 ng/ml for all Other substances. Besides, methods have been optimized for the hydrolysis of the glucuronic acid conjugated metabolites. This specific detection method is useful, as polymedication is a usual practice in clinical situations, and its sensitivity allows its use for single-dose pharmacokinetic studies.

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