Rec 15/2615
Rec 15/2615 is a potent α(1)-adrenergic agonist that stimulates small cholangiocyte proliferation through the activation of calcium-dependent signaling pathways.
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- CAS. Nr.: 173059-47-7
- Formel: C26H33N5O5
- Molecular Weight:495.57
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
Beschreibung
Chemical Information
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CAS. Nr. 173059-47-7
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Molecular Weight 495.57
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Formel C26H33N5O5
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SMILES
O=C(COC1=C(C(C)C)C=CC=C1OC)N2CCN(CC2)C3=NC4=C(C=C(C(OC)=C4)OC)C(N)=N3
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Protokoll
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)