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  4. Casein Kinase I alpha Protein, Human (sf9, GST)

Casein Kinase I alpha Protein, Human (sf9, GST)

Cat. No.: HY-P72872
Handling Instructions

Casein kinase I α is a member of casein kinases that exhibits a preference for phosphorylation of acidic proteins such as casein. It phosphorylates many substrates, including CTNNB1 at "Ser-45" involved in Wnt signaling. Casein Kinase I alpha Protein, Human (sf9, GST) is the recombinant human-derived Casein Kinase I alpha protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of Casein Kinase I alpha Protein, Human (sf9, GST) is 337 a.a., with molecular weight of ~62 kDa.

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Description

Casein kinase I α is a member of casein kinases that exhibits a preference for phosphorylation of acidic proteins such as casein. It phosphorylates many substrates, including CTNNB1 at "Ser-45" involved in Wnt signaling. Casein Kinase I alpha Protein, Human (sf9, GST) is the recombinant human-derived Casein Kinase I alpha protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of Casein Kinase I alpha Protein, Human (sf9, GST) is 337 a.a., with molecular weight of ~62 kDa.

Background

Casein Kinase I alpha, a member of the casein kinase family, is characterized by its preference for acidic substrates such as caseins. Demonstrating versatility, it phosphorylates a diverse array of proteins and actively participates in Wnt signaling by phosphorylating CTNNB1 at 'Ser-45.' Additionally, Casein Kinase I alpha may contribute to the phosphorylation of PER1 and PER2, suggesting a potential role in circadian rhythm regulation. Beyond its involvement in cellular signaling pathways, this kinase may play a crucial role in chromosome segregation during mitosis and regulate epithelial cell migration by influencing keratin cytoskeleton disassembly. Furthermore, Casein Kinase I alpha acts as a positive regulator of mTORC1 and mTORC2 signaling in response to nutrient stimuli, mediating the phosphorylation of the DEPTOR inhibitor. In another context, it functions as an inhibitor of NLRP3 inflammasome assembly through the phosphorylation of NLRP3, highlighting its diverse roles in cellular processes.

Species

Human

Source

Sf9 insect cells

Tag

N-GST

Accession

P48729-1 (M1-F337)

Gene ID
Molecular Construction
N-term
GST
CSNK1A1 (M1-F337)
Accession # P48729-1
C-term
Synonyms
Casein kinase I isoform alpha; CKI-alpha; CK1; CSNK1A1
AA Sequence

MASSSGSKAEFIVGGKYKLVRKIGSGSFGDIYLAINITNGEEVAVKLESQKARHPQLLYESKLYKILQGGVGIPHIRWYGQEKDYNVLVMDLLGPSLEDLFNFCSRRFTMKTVLMLADQMISRIEYVHTKNFIHRDIKPDNFLMGIGRHCNKLFLIDFGLAKKYRDNRTRQHIPYREDKNLTGTARYASINAHLGIEQSRRDDMESLGYVLMYFNRTSLPWQGLKAATKKQKYEKISEKKMSTPVEVLCKGFPAEFAMYLNYCRGLRFEEAPDYMYLRQLFRILFRTLNHQYDYTFDWTMLKQKAAQQAASSSGQGQQAQTPTGKQTDKTKSNMKGF

Molecular Weight

Approximately 62 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Casein Kinase I alpha Protein, Human (sf9, GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Casein Kinase I alpha Protein, Human (sf9, GST)
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HY-P72872
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