Ephrin-B2/EFNB2 Protein, Human (HEK293, His)
Based on 1 Customer Validation
Ephrin-B2/EFNB2 is a key transmembrane ligand of Eph receptors that coordinates migration, repulsion, and adhesion in developing neurons, blood vessels, and epithelia. It induces bidirectional signaling, binding to EPHA4, EPHA3 and EPHB4, which is critical for cardiac morphogenesis and angiogenesis. Ephrin-B2/EFNB2 Protein, Human (HEK293, His) is the recombinant human-derived Ephrin-B2/EFNB2 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Ephrin-B2/EFNB2 is a key transmembrane ligand of Eph receptors that coordinates migration, repulsion, and adhesion in developing neurons, blood vessels, and epithelia. It induces bidirectional signaling, binding to EPHA4, EPHA3 and EPHB4, which is critical for cardiac morphogenesis and angiogenesis. Ephrin-B2/EFNB2 Protein, Human (HEK293, His) is the recombinant human-derived Ephrin-B2/EFNB2 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
Ephrin-B2, also recognized as EFNB2, emerges as a pivotal cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases critical in orchestrating migration, repulsion, and adhesion during neuronal, vascular, and epithelial development. Displaying a propensity to bind promiscuously to Eph receptors on adjacent cells, Ephrin-B2 instigates contact-dependent bidirectional signaling, delineated into forward signaling downstream of the receptor and reverse signaling downstream of the ephrin ligand. Its binding affinity extends to receptor tyrosine kinases, including EPHA4, EPHA3, and EPHB4, with the latter forming a crucial partnership in heart morphogenesis and angiogenesis, governing cell adhesion and migration. In EPHB4-mediated forward signaling, Ephrin-B2 regulates cellular repulsion and segregation from EFNB2-expressing cells, potentially influencing the orientation of longitudinally projecting axons. Notably, Ephrin-B2 assumes a unique role as a receptor for Hendra virus and Nipah virus during microbial infection, adding an intriguing facet to its multifaceted functions.
Verified Bioactivity
Immobilized Recombinant Nipah Virus G Protein at 2 μg/mL (100 μL/well) can bind Recombinant Human EFNB2 (C-6His). Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Human EFNB2 (C-6His) is 6.68 ng/mL.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
P52799 (I28-A229)
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Molecular Construction
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N-term
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EFNB2 (I28-A229)
Accession # P52799 -
6*His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
EFNB2; Eph-Related Receptor Tyrosine Kinase Ligand 5; Prev. EPLG5; MGC126226; Ephrin-B2; MGC126227; LERK5; MGC126228; HTKL; EPH-Related Receptor Tyrosine Kinase Ligand 5; HTK Ligand; LERK-5; Htk-L; HTK-L; Ephrin B2; Ligand Of Eph-Related Kinase 5
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AA Sequence
IVLEPIYWNSSNSKFLPGQGLVLYPQIGDKLDIICPKVDSKTVGQYEYYKVYMVDKDQADRCTIKKENTPLLNCAKPDQDIKFTIKFQEFSPNLWGLEFQKNKDYYIISTSNGSLEGLDNQEGGVCQTRAMKILMKVGQDASSAGSTRNKDPTRRPELEAGTNGRSSTTSPFVKPNPGSSTDGNSAGHSGNNILGSEVALFA
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Predicted Molecular Mass
23.2 kDa
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Molecular Weight
Approximately 30-40 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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