FGF-1 Protein, Human (154a.a)

FGF-1 Protein intricately regulates cell survival, division, angiogenesis, differentiation, and migration. Acting as a potent mitogen, it serves as a ligand for FGFR1 and integrins. Binding to FGFR1, especially with heparin, leads to dimerization and activation, initiating signaling cascades. Additionally, FGF-1 Protein binds to integrin ITGAV:ITGB3, forming a ternary complex crucial for FGF1 signaling. Inducing phosphorylation of key proteins, it plays a multifaceted role in diverse cellular processes, emphasizing its significance in cell physiology. FGF-1 Protein, Human (154a.a) is the recombinant human-derived FGF-1 protein, expressed by E. coli, with His, tag free. The total length of FGF-1 Protein, Human (154a.a) is 154 a.a., with molecular weight of 18.54 kDa.

FGF-1 Protein assumes a pivotal role in intricately regulating cell survival, division, angiogenesis, differentiation, and migration. Functioning as a potent mitogen in vitro, it acts as a ligand for FGFR1 and integrins. Binding to FGFR1, particularly in the presence of heparin, leads to FGFR1 dimerization and activation through sequential autophosphorylation on tyrosine residues. This activation serves as docking sites for interacting proteins, initiating several signaling cascades. Furthermore, FGF-1 Protein binds to integrin ITGAV:ITGB3, forming a ternary complex with integrin and FGFR1, crucial for FGF1 signaling. Inducing the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2, and AKT1, it demonstrates its involvement in diverse cellular processes. Additionally, FGF-1 Protein's ability to induce angiogenesis further underscores its multifaceted role. Interactions with FGFRs, integrins, and various proteins within complex networks highlight its intricate participation in cellular signaling pathways, emphasizing its significance in cell physiology.

Human

E. coli

Tag Free

P05230 (A2-D155)

2246  [NCBI]

AEGEITTFTALTEKFNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD

18.54 KDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.