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  2. Receptor Proteins
  3. G-Protein-Coupled Receptors (GPCRs)
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  5. Frizzled-5
  6. Frizzled-5 Protein, Human (HEK293, Fc)

Frizzled-5 Protein, Human (HEK293, Fc)

Cat. No.: HY-P74139
Handling Instructions

Frizzled-5 protein is a receptor for Wnt proteins (WNT2, WNT10B, WNT5A), which can activate WNT7A and promote the formation of synapses in neurons. It participates in the canonical Wnt/β-catenin pathway, activating disheveled proteins, inhibiting GSK-3 kinase, and triggering Wnt target genes. Frizzled-5 Protein, Human (HEK293, Fc) is the recombinant human-derived Frizzled-5 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Frizzled-5 Protein, Human (HEK293, Fc) is 141 a.a., with molecular weight of ~42.7 kDa.

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Description

Frizzled-5 protein is a receptor for Wnt proteins (WNT2, WNT10B, WNT5A), which can activate WNT7A and promote the formation of synapses in neurons. It participates in the canonical Wnt/β-catenin pathway, activating disheveled proteins, inhibiting GSK-3 kinase, and triggering Wnt target genes. Frizzled-5 Protein, Human (HEK293, Fc) is the recombinant human-derived Frizzled-5 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Frizzled-5 Protein, Human (HEK293, Fc) is 141 a.a., with molecular weight of ~42.7 kDa.

Background

Frizzled-5 Protein functions as a receptor for Wnt proteins, including WNT2, WNT10B, and WNT5A, although it does not activate WNT2B or WNT4 in vitro, and the in vivo context may vary due to potential differences in coexpression. In neurons, the activation of WNT7A by Frizzled-5 promotes synapse formation. It actively participates in the canonical Wnt/beta-catenin signaling pathway, leading to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin, and subsequent activation of Wnt target genes. Frizzled-5 may also engage a secondary signaling pathway involving PKC and calcium fluxes, the integration of which with the canonical pathway remains unclear, given PKC's apparent requirement for Wnt-mediated inactivation of GSK-3 kinase. This versatile receptor likely plays a role in transduction and intercellular transmission of polarity information during tissue morphogenesis and in differentiated tissues. Additionally, Frizzled-5 is implicated in yolk sac angiogenesis and placental vascularization, and its homodimerization is promoted by the binding of unsaturated fatty acid molecules via the FZ domain. Notably, Frizzled-5 interacts with WNT2B, WNT7A, and GOPC, contributing to its diverse range of cellular functions.

Species

Human

Source

HEK293

Tag

C-hFc

Accession

Q13467 (A27-P167)

Gene ID
Molecular Construction
N-term
Frizzled-5 (A27-P167)
Accession # Q13467
hFc
C-term
Synonyms
C2orf31; Frizzled homolog 5 (Drosophila); Frizzled-5; FZ-5; FZD5
Molecular Weight

Approximately 42.7 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Frizzled-5 Protein, Human (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Frizzled-5 Protein, Human (HEK293, Fc)
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HY-P74139
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