1. Recombinant Proteins
  2. Receptor Proteins
  3. G-Protein-Coupled Receptors (GPCRs)
  4. Frizzled
  5. Frizzled-5
  6. Frizzled-5 Protein, Mouse (CHO, Fc)

Frizzled-5 Protein, Mouse (CHO, Fc)

Cat. No.: HY-P74136
COA Handling Instructions

Frizzled-5 is a Wnt receptor that activates canonical Wnt/β-catenin signaling through WNT2, WNT10B, and WNT5A. It participates in a potential second pathway involving PKC and calcium flux, affecting Wnt-mediated inactivation of GSK-3 kinase. Frizzled-5 Protein, Mouse (CHO, Fc) is the recombinant mouse-derived Frizzled-5 protein, expressed by CHO , with C-hFc labeled tag.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
50 μg $350 In-stock
100 μg $600 In-stock
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Description

Frizzled-5 is a Wnt receptor that activates canonical Wnt/β-catenin signaling through WNT2, WNT10B, and WNT5A. It participates in a potential second pathway involving PKC and calcium flux, affecting Wnt-mediated inactivation of GSK-3 kinase. Frizzled-5 Protein, Mouse (CHO, Fc) is the recombinant mouse-derived Frizzled-5 protein, expressed by CHO , with C-hFc labeled tag.

Background

Frizzled-5, a receptor for Wnt proteins, demonstrates a nuanced activation profile, being responsive to WNT2, WNT10B, and WNT5A, while showing no affinity for WNT2B or WNT4 in vitro. Its involvement in canonical Wnt/beta-catenin signaling is pivotal, leading to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin, and subsequent activation of Wnt target genes. Additionally, a second signaling pathway, potentially involving PKC and calcium fluxes, adds complexity, with implications for Wnt-mediated inactivation of GSK-3 kinase. Frizzled-5's engagement in tissue morphogenesis and potential participation in transducing polarity information underscores its versatile role. Notably, in neurons, WNT7A activation facilitates synapse formation. Its contributions extend to yolk sac angiogenesis and placental vascularization. Homodimerization, facilitated by binding of unsaturated fatty acid molecules, and interactions with WNT2B, WNT7A, and GOPC further underscore the multifaceted regulatory functions of Frizzled-5 within intricate signaling networks.

Species

Mouse

Source

CHO

Tag

C-hFc

Accession

Q9EQD0 (A27-P167)

Gene ID
Molecular Construction
N-term
Frizzled-5 (A27-P167)
Accession # Q9EQD0
hFc
C-term
Synonyms
Frizzled-5; Fz-5; mFz5; Fzd5
Molecular Weight

52-56 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Frizzled-5 Protein, Mouse (CHO, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Frizzled-5 Protein, Mouse (CHO, Fc)
Cat. No.:
HY-P74136
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