IL-12 Protein, Human (Biotinylated, HEK293, His-Avi)

IL-35 Protein plays a pivotal role in immune regulation, forming the IL-12 cytokine with IL12B or the IL-35 cytokine with EBI3/IL27B. IL-12 regulates T-cell and natural killer-cell responses, inducing interferon-gamma production. IL-35 contributes significantly to maintaining immune homeostasis in the liver, acting as an immune-suppressive cytokine. Mechanistically, IL-12 exerts effects through the IL12R1 and IL12R2 receptor subunits, phosphorylating cellular substrates and regulating cytokine-responsive genes via phosphorylated STAT4. In the IL-35 context, unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers mediate signaling, requiring transcription factors STAT1 and STAT4. IL-35 interacts with NBR1, promoting IL-12 secretion, and the IL-35 heterodimer with EBI3/IL27B is non-disulfide-linked, distinguishing it from IL-12. IL-12 Protein, Human (Biotinylated, HEK293, His-Avi) is a recombinant protein dimer complex containing human-derived IL-12 protein, expressed by HEK293, with C-Avi, C-His labeled tag. IL-12 Protein, Human (Biotinylated, HEK293, His-Avi), has molecular weight of ~40-45 kDa.

IL-35 Protein plays a pivotal role in immune regulation, exhibiting versatility in its functions. It heterodimerizes with IL12B to form the IL-12 cytokine or with EBI3/IL27B to create the IL-35 cytokine. IL-12, primarily produced by professional antigen-presenting cells such as B-cells, dendritic cells, macrophages, and granulocytes, serves as a crucial link between innate resistance and adaptive immunity, regulating T-cell and natural killer-cell responses while inducing interferon-gamma production and favoring the differentiation of T-helper 1 cells. Mechanistically, IL-12 exerts its effects through a receptor composed of IL12R1 and IL12R2 subunits, leading to tyrosine phosphorylation of cellular substrates and subsequent regulation of cytokine/growth factor responsive genes by recruited phosphorylated STAT4. In the context of IL-35, IL-35 contributes significantly to maintaining immune homeostasis in the liver microenvironment and functions as an immune-suppressive cytokine. Notably, IL-35 mediates its effects through unconventional receptors composed of IL12RB2 and gp130/IL6ST heterodimers or homodimers, requiring the transcription factors STAT1 and STAT4 for signaling. Additionally, IL-35 interacts with NBR1, promoting IL-12 secretion. The IL-35 heterodimer with EBI3/IL27B, known as interleukin IL-35, is not disulfide-linked, distinguishing it from the disulfide-linked IL-12 heterodimer with IL12B.

Human

HEK293

C-Avi;C-His

P29459 (R23-S219) & P29460 (I23-S328)

3592  [NCBI]&3593  [NCBI]

Approximately 40-45 kDa

Greater than 95% as determined by Tris-Bis PAGE.

<1 EU/μg, determined by LAL method.