1. Recombinant Proteins
  2. Cytokines and Growth Factors CD Antigens
  3. Interleukin & Receptors NK Cell CD Proteins
  4. IL-12 Receptor IL-12R beta 1/CD212
  5. IL-12R beta 2
  6. IL-12R beta 2 Protein, Mouse (HEK293, His)

IL-12R beta 2 Protein, Mouse (HEK293, His)

Cat. No.: HY-P74834
COA Handling Instructions

IL-12R beta 2 protein is an IL-12 cytokine surface receptor that activates the Jak-Stat signaling cascade pathway and is involved in IL-12-mediated immune regulation. IL-12R beta 2 Protein, Mouse (HEK293, His) is expressed by HEK 293 cells with a transmembrane region (W638-I658) and a His tag at the C-terminus.

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Description

IL-12R beta 2 protein is an IL-12 cytokine surface receptor that activates the Jak-Stat signaling cascade pathway and is involved in IL-12-mediated immune regulation. IL-12R beta 2 Protein, Mouse (HEK293, His) is expressed by HEK 293 cells with a transmembrane region (W638-I658) and a His tag at the C-terminus[1].

Background

IL-12 receptor is a type I cytokine receptor that binds to IL-12. IL-12 receptor is expressed primarily on NK and T cells and consists of beta 1 and beta 2 subunits and is a member of the gp130 cytokine receptor superfamily. IL-12R beta 2 (abbreviated IL-12R2 or IL-12Rβ2) is a subunit of the IL-12 receptor, and IL-12Rβ2 is its human gene name. Co-expression of IL-12Rβ2 protein and IL-12Rβ1 protein can lead to the formation of high-affinity IL-12 binding sites and the reconstitution of IL-12-dependent signaling. Upon IL-12 binding to the IL-12 receptor, the cytoplasmic protein TYK2, which interacts directly with IL-12Rβ1, and JAK2, which interacts with IL-12Rβ2, are tyrosine phosphorylated. Phosphorylated TYK2 and JAK2 are required for subsequent tyrosine phosphorylation and activation of STAT4 that binds to IL-12Rβ2. STAT4 is a transcription factor that subsequently homodimerizes, translocates to the nucleus and binds to its target DNA to activate transcription of IFN-γ and other target genes[1].
Expression of the IL-12Rβ2 chain is thought to be a key factor in controlling T cell development and function. It plays an important role in Th1 cell differentiation and deletion results in failure of Th1 differentiation and dysfunctional production of Th1 effector molecules. Up-regulation of this gene has been found to be associated with many infectious diseases, such as Crohn's disease and leprosy, which are thought to contribute to the inflammatory response and host defense[2].

In Vivo

The number of Treg cells in the resting spleen of IL-12Rβ2-/- mice is comparable to that of strain-matched wild-type (WT) mice. Among them, IL-12Rβ2-/- splenocytes are completely resistant to IL-12-mediated inhibition and lacked detectable IL-12 receptor mRNA in reg cells, suggesting that IL-12 signaling can indirectly inhibit Treg amplification[3].
IL-12Rβ2 is expressed primarily in NK cells, and mice lacking the IL-12Rβ2 subunit exhibit transient weight loss, the severity of which correlates with serum cytokine levels of IFN-γ. And, serum levels of IL-1β and IL-23 can be detected in IL-12Rβ-/-.Rag1-/-mice on C57BL/6 background, even in the absence of anti-CD4 treatment. IL-12Rβ2-/- .Rag1-/- mice exhibits elevated mRNA levels of most pro-inflammatory cytokines[4].

Biological Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse IL‑12R beta 2 is immobilized at 0.5 μg/mL (100 μL/well), the concentration of rmIL-12 that produces 50% of the optimal binding response is found to be approximately 19.97 ng/mL.

  • Measured by its binding ability in a functional ELISA. When Recombinant Mouse IL‑12R beta 2 is immobilized at 0.5 μg/mL (100 μL/well), the concentration of rmIL-12 that produces 50% of the optimal binding response is found to be approximately 19.97 ng/mL.
Species

Mouse

Source

HEK293

Tag

C-His

Accession

P97378 (N24-N637)

Gene ID
Molecular Construction
N-term
IL-12Rβ2 (N24-N637)
Accession # P97378
His
C-term
Synonyms
Interleukin-12 receptor subunit beta-2; IL-12R-beta-2; IL-12RB2
AA Sequence

NIDVCKLGTVTVQPAPVIPLGSAANISCSLNPKQGCSHYPSSNELILLKFVNDVLVENLHGKKVHDHTGHSSTFQVTNLSLGMTLFVCKLNCSNSQKKPPVPVCGVEISVGVAPEPPQNISCVQEGENGTVACSWNSGKVTYLKTNYTLQLSGPNNLTCQKQCFSDNRQNCNRLDLGINLSPDLAESRFIVRVTAINDLGNSSSLPHTFTFLDIVIPLPPWDIRINFLNASGSRGTLQWEDEGQVVLNQLRYQPLNSTSWNMVNATNAKGKYDLRDLRPFTEYEFQISSKLHLSGGSWSNWSESLRTRTPEEEPVGILDIWYMKQDIDYDRQQISLFWKSLNPSEARGKILHYQVTLQEVTKKTTLQNTTRHTSWTRVIPRTGAWTASVSAANSKGASAPTHINIVDLCGTGLLAPHQVSAKSENMDNILVTWQPPKKADSAVREYIVEWRALQPGSITKFPPHWLRIPPDNMSALISENIKPYICYEIRVHALSESQGGCSSIRGDSKHKAPVSGPHITAITEKKERLFISWTHIPFPEQRGCILHYRIYWKERDSTAQPELCEIQYRRSQNSHPISSLQPRVTYVLWMTAVTAAGESPQGNEREFCPQGKAN

Molecular Weight

100-130 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
IL-12R beta 2 Protein, Mouse (HEK293, His)
Cat. No.:
HY-P74834
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