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IL-13R alpha 2 Protein, Human (HEK293, His)

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Based on 1 Customer Validation

IL-13R alpha 2 Protein, functioning as a monomer, exhibits high-affinity binding exclusively to interleukin-13 (IL13) without interaction with interleukin-4 (IL4). This binding selectivity indicates a unique role and specificity for IL-13R alpha 2 in mediating cellular responses to IL13, emphasizing its importance in IL13-associated cellular processes. IL-13R alpha 2 Protein, Human (HEK293, His) is the recombinant human-derived IL-13R alpha 2 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for IL-13R alpha 2 Protein, Human (HEK293, His)
  • Experimental Validation Results for IL-13R alpha 2 Protein, Human (HEK293, His)
  • IL-13R alpha 2 Protein, Human (HEK293, His) Product Image
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Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

IL-13R alpha 2 Protein, functioning as a monomer, exhibits high-affinity binding exclusively to interleukin-13 (IL13) without interaction with interleukin-4 (IL4). This binding selectivity indicates a unique role and specificity for IL-13R alpha 2 in mediating cellular responses to IL13, emphasizing its importance in IL13-associated cellular processes. IL-13R alpha 2 Protein, Human (HEK293, His) is the recombinant human-derived IL-13R alpha 2 protein, expressed by HEK293 , with C-His labeled tag.

Background

The IL-13R alpha 2 protein functions as a monomer with high affinity, specifically binding to interleukin-13 (IL13) but not to interleukin-4 (IL4). This selectivity in binding suggests a distinct role and specificity for IL-13R alpha 2 in mediating responses to IL13, highlighting its importance in cellular processes associated with this cytokine.

Verified Bioactivity

Measured by its ability to inhibit IL-13-dependent proliferation of TF-1 human erythroleukemic cells.The ED50 for this effect is 0.02-0.2 μg/mL in the presence of 8 ng/mL recombinant human IL-13 (HY-P70568).

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit IL-13-dependent proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.053 μg/mL in the presence of 8 ng/mL recombinant human IL-13, corresponding to a specific activity is 1.880×104 U/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    Q14627/NP_000631.1 (D27-L342)

  • Gene ID
    3598  [NCBI]
  • Molecular Construction
    • N-term
    • IL-13Rα2 (D27-L342)
      Accession # Q14627/NP_000631.1
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    IL13RA2; IL-13R Subunit Alpha-2; Interleukin 13 Receptor Subunit Alpha 2; IL-13R-Alpha-2; CD213a2; IL-13RA2; IL-13R; Interleukin-13 Receptor Alpha 2 Variant; IL13BP; Interleukin 13 Receptor Alpha 2 Chain; CT19; Interleukin 13 Binding Protein; Interleukin-13 Receptor Subunit Alpha-2; Interleukin-13-Binding Protein; Interleukin 13 Receptor, Alpha 2; CD213a2 Antigen; IL-13 Receptor Subunit Alpha-2; IL13R; Cancer/Testis Antigen 19

  • AA Sequence

    DTEIKVNPPQDFEIVDPGYLGYLYLQWQPPLSLDHFKECTVEYELKYRNIGSETWKTIITKNLHYKDGFDLNKGIEAKIHTLLPWQCTNGSEVQSSWAETTYWISPQGIPETKVQDMDCVYYNWQYLLCSWKPGIGVLLDTNYNLFYWYEGLDHALQCVDYIKADGQNIGCRFPYLEASDYKDFYICVNGSSENKPIRSSYFTFQLQNIVKPLPPVYLTFTRESSCEIKLKWSIPLGPIPARCFDYEIEIREDDTTLVTATVENETYTLKTTNETRQLCFVVRSKVNIYCSDDGIWSEWSDKQCWEGEDLSKKTLL

  • Molecular Weight

    Approximately 48-58 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit IL-13-dependent proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.053 μg/mL in the presence of 8 ng/mL recombinant human IL-13, corresponding to a specific activity is 1.880×104 U/mg.

  • IL-13R alpha 2 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for IL-13R alpha 2 Protein, Human (HEK293, His)
  • Experimental Validation Results for IL-13R alpha 2 Protein, Human (HEK293, His)
  • IL-13R alpha 2 Protein, Human (HEK293, His) Product Image