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MFG-E8 Protein, Human (Biotinylated, sf9, His-Avi)

Cat. No.: HY-P73816
Handling Instructions

MFG-E8 Protein plays a crucial role in maintaining intestinal epithelial homeostasis, facilitating mucosal healing, and contributing to VEGF-dependent neovascularization. It acts as a specific ligand, binding to alpha-v/beta-3 and alpha-v/beta-5 receptors, and participates in the phagocytic removal of apoptotic cells. The protein is also implicated in zona pellucida binding and is a major component of aortic medial amyloid. MFG-E8 Protein, Human (Biotinylated, sf9, His-Avi) is the recombinant human-derived MFG-E8 protein, expressed by Sf9 insect cells , with C-Avi, C-His labeled tag. The total length of MFG-E8 Protein, Human (Biotinylated, sf9, His-Avi) is 387 a.a., with molecular weight of ~44.1 kDa.

For research use only. We do not sell to patients.

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Description

MFG-E8 Protein plays a crucial role in maintaining intestinal epithelial homeostasis, facilitating mucosal healing, and contributing to VEGF-dependent neovascularization. It acts as a specific ligand, binding to alpha-v/beta-3 and alpha-v/beta-5 receptors, and participates in the phagocytic removal of apoptotic cells. The protein is also implicated in zona pellucida binding and is a major component of aortic medial amyloid. MFG-E8 Protein, Human (Biotinylated, sf9, His-Avi) is the recombinant human-derived MFG-E8 protein, expressed by Sf9 insect cells , with C-Avi, C-His labeled tag. The total length of MFG-E8 Protein, Human (Biotinylated, sf9, His-Avi) is 387 a.a., with molecular weight of ~44.1 kDa.

Background

The MFG-E8 protein is involved in multiple essential functions, including the maintenance of intestinal epithelial homeostasis and the facilitation of mucosal healing. It aids in VEGF-dependent neovascularization and contributes to the phagocytic removal of apoptotic cells in various tissues. Acting as a specific ligand, it binds to the alpha-v/beta-3 and alpha-v/beta-5 receptors, and it also has the ability to bind to cell surfaces enriched with phosphatidylserine, independently of receptors. Furthermore, it serves as a zona pellucida-binding protein that potentially plays a role in gamete interaction. Additionally, the MFG-E8 protein is a main constituent of aortic medial amyloid.

Species

Human

Source

Sf9 insect cells

Tag

C-Avi;C-His

Accession

Q08431 (M1-C387)

Gene ID
Molecular Construction
N-term
MFG-E8 (M1-C387)
Accession # Q08431
Avi-His
C-term
Synonyms
Lactadherin; HMFG; MFGM; MFG-E8; SED1
Molecular Weight

Approximately 44.1 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

MFG-E8 Protein, Human (Biotinylated, sf9, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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MFG-E8 Protein, Human (Biotinylated, sf9, His-Avi)
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HY-P73816
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