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OTUD5 Protein, Human (His, FLAG)

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The OTUD5 protein is a deubiquitinating enzyme that crucially eliminates the innate immune response through peptidase activity on "Lys-48" and "Lys-63" linked polyubiquitin chains, thereby inhibiting TRAF3-mediated Type I interferon production. It acts as a regulator of neuroectodermal differentiation by cleaving “Lys-48” linked ubiquitin chains to prevent the degradation of chromatin regulators such as ARID1A, HDAC2 and HCF1. OTUD5 Protein, Human (His, FLAG) is the recombinant human-derived OTUD5 protein, expressed by E. coli , with N-6*His, N-Flag labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • OTUD5 Protein, Human (His, FLAG) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The OTUD5 protein is a deubiquitinating enzyme that crucially eliminates the innate immune response through peptidase activity on "Lys-48" and "Lys-63" linked polyubiquitin chains, thereby inhibiting TRAF3-mediated Type I interferon production. It acts as a regulator of neuroectodermal differentiation by cleaving “Lys-48” linked ubiquitin chains to prevent the degradation of chromatin regulators such as ARID1A, HDAC2 and HCF1. OTUD5 Protein, Human (His, FLAG) is the recombinant human-derived OTUD5 protein, expressed by E. coli , with N-6*His, N-Flag labeled tag.

Background

OTUD5 Protein, a deubiquitinating enzyme, plays a crucial role as a negative regulator of the innate immune system, exhibiting peptidase activity towards 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, and the ability to cleave 'Lys-11'-linked ubiquitin chains in vitro. It exerts its influence through TRAF3 deubiquitination, subsequently suppressing type I interferon (IFN) production. Additionally, OTUD5 serves as a key controller of neuroectodermal differentiation, counteracting the degradation of essential chromatin regulators like ARID1A, HDAC2, and HCF1 by cleaving 'Lys-48'-linked ubiquitin chains. Furthermore, following phosphorylation by MTOR, OTUD5 acts as a positive regulator of mTORC1 and mTORC2 signaling, mediating the deubiquitination of BTRC and enhancing its stability. This multifaceted role underscores the significance of OTUD5 in modulating diverse cellular processes, highlighting its pivotal contributions to immune regulation, cellular differentiation, and signaling pathways.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-His;C-Flag
  • Accession

    Q96G74 (G172-G351)

  • Gene ID
    55593
  • Molecular Construction
    • N-term
    • 6*His
    • OTUD5 (G172-G351)
      Accession # Q96G74
    • Flag
    • C-term
  • Protein Length

    Partial

  • Synonyms

    OTUD5; OTU Domain Containing 5; OTU Deubiquitinase 5; Deubiquitinase A; DUBA; DKFZp761A052; OTU Domain-Containing Protein 5; Deubiquinating Enzyme A; Deubiquitinating Enzyme A; MCAND

  • AA Sequence

    GAGYNSEDEYEAAAARIEAMDPATVEQQEHWFEKALRDKKGFIIKQMKEDGACLFRAVADQVYGDQDMHEVVRKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEMYNRPVEVYQYSTGTSAVEPINTFHGIHQNEDEPIRVSYHRNIHYNSVVNPNKATIGVGLG

  • Predicted Molecular Mass

    23.9 kDa

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of PBS.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
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Biological Activity (ED50) Biological Activity (ED50)
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ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • OTUD5 Protein, Human (His, FLAG) Product Image