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Prolactin Receptor Protein, Human (HEK293, His)

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Based on 1 Customer Validation

The Prolactin Receptor Protein is the receptor for prolactin, encoded by this gene, and promotes prolactin-dependent signaling through ligand-induced dimerization. This gene exhibits multiple splice variants, encodes multiple isoforms, and has a potential role in regulating the endocrine and autocrine effects of prolactin. Prolactin Receptor Protein, Human (HEK293, His) is the recombinant human-derived Prolactin Receptor Protein, expressed by HEK293 , with C-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Prolactin Receptor Protein, Human (HEK293, His)
  • Experimental Validation Results for Prolactin Receptor Protein, Human (HEK293, His)
  • Prolactin Receptor Protein, Human (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The Prolactin Receptor Protein is the receptor for prolactin, encoded by this gene, and promotes prolactin-dependent signaling through ligand-induced dimerization. This gene exhibits multiple splice variants, encodes multiple isoforms, and has a potential role in regulating the endocrine and autocrine effects of prolactin. Prolactin Receptor Protein, Human (HEK293, His) is the recombinant human-derived Prolactin Receptor Protein, expressed by HEK293 , with C-10*His labeled tag.

Background

Prolactin Receptor Protein, encoded by this gene, is a receptor for the anterior pituitary hormone prolactin and is a member of the type I cytokine receptor family. Its function involves prolactin-dependent signaling, triggered by ligand-induced dimerization of the prolactin receptor. The gene exhibits several alternatively spliced transcript variants encoding diverse membrane-bound and soluble isoforms, potentially contributing to the modulation of the endocrine and autocrine effects of prolactin in both normal tissue and cancer. The expression of Prolactin R demonstrates bias, with notable levels observed in the placenta (RPKM 18.9), endometrium (RPKM 11.6), and eight other tissues, underscoring its specialized role in various physiological contexts and its potential involvement in reproductive and endocrine processes.

Verified Bioactivity

1.Measured by its ability to inhibit Prolactin-induced proliferation of Nb2-11 rat lymphoma cells. The ED50 this effect is 0.03362-0.095 μg/mL in the presence of 0.5 ng/mL of recombinant human Prolactin.
2.Human Prolactin Receptor captured on CM5 Chip can bind Human Prolactin（HY-P73380）with an affinity constant of 1.0-5.0 nM as determined in SPR assay.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit Prolactin-induced proliferation of Nb2‑11 rat lymphoma cells. The ED50 this effect is 0.03362 μg/mL in the presence of 0.5 ng/mL of recombinant human Prolactin, corresponding to a specific activity is 2.97×104 units/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-10*His
  • Accession

    NP_000940.1 (Q25-D234)

  • Gene ID
    5618  [NCBI]
  • Molecular Construction
    • N-term
    • Prolactin R (Q25-D234)
      Accession # NP_000940.1
    • 10*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    PRLR; HPRLrI; Prolactin Receptor; PRL-R; Secreted Prolactin Binding Protein; MFAB; HPRL Receptor; HPRL; RI-PRLR

  • AA Sequence

    QLPPGKPEIFKCRSPNKETFTCWWRPGTDGGLPTNYSLTYHREGETLMHECPDYITGGPNSCHFGKQYTSMWRTYIMMVNATNQMGSSFSDELYVDVTYIVQPDPPLELAVEVKQPEDRKPYLWIKWSPPTLIDLKTGWFTLLYEIRLKPEKAAEWEIHFAGQQTEFKILSLHPGQKYLVQVRCKPDHGYWSAWSPATFIQIPSDFTMND

  • Molecular Weight

    Approximately 36-38 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, 5-8% trehalose, 0.01% Tween 80, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit Prolactin-induced proliferation of Nb2‑11 rat lymphoma cells. The ED50 this effect is 0.03362 μg/mL in the presence of 0.5 ng/mL of recombinant human Prolactin, corresponding to a specific activity is 2.97×104 units/mg.

  • Prolactin Receptor Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Prolactin Receptor Protein, Human (HEK293, His)
  • Experimental Validation Results for Prolactin Receptor Protein, Human (HEK293, His)
  • Prolactin Receptor Protein, Human (HEK293, His) Product Image