Prolactin R Protein, Human (HEK293, His)

The prolactin R protein is the receptor for prolactin, encoded by this gene, and promotes prolactin-dependent signaling through ligand-induced dimerization. This gene exhibits multiple splice variants, encodes multiple isoforms, and has a potential role in regulating the endocrine and autocrine effects of prolactin. Prolactin R Protein, Human (HEK293, His) is the recombinant human-derived Prolactin R protein, expressed by HEK293 , with C-10*His labeled tag.

Prolactin R Protein, encoded by this gene, is a receptor for the anterior pituitary hormone prolactin and is a member of the type I cytokine receptor family. Its function involves prolactin-dependent signaling, triggered by ligand-induced dimerization of the prolactin receptor. The gene exhibits several alternatively spliced transcript variants encoding diverse membrane-bound and soluble isoforms, potentially contributing to the modulation of the endocrine and autocrine effects of prolactin in both normal tissue and cancer. The expression of Prolactin R demonstrates bias, with notable levels observed in the placenta (RPKM 18.9), endometrium (RPKM 11.6), and eight other tissues, underscoring its specialized role in various physiological contexts and its potential involvement in reproductive and endocrine processes.

Measured by its ability to inhibit Prolactin-induced proliferation of Nb2-11 rat lymphoma cells. The ED₅₀ this effect is 0.03362-0.095 μg/mL in the presence of 0.5 ng/mL of recombinant human Prolactin.

• 
  Measured by its ability to inhibit Prolactin-induced proliferation of Nb2‑11 rat lymphoma cells. The ED₅₀ this effect is 0.03362 μg/mL in the presence of 0.5 ng/mL of recombinant human Prolactin, corresponding to a specific activity is 2.97×10⁴ units/mg.

Human

HEK293

C-10*His

NP_000940.1 (Q25-D234)

5618  [NCBI]

Partial

QLPPGKPEIFKCRSPNKETFTCWWRPGTDGGLPTNYSLTYHREGETLMHECPDYITGGPNSCHFGKQYTSMWRTYIMMVNATNQMGSSFSDELYVDVTYIVQPDPPLELAVEVKQPEDRKPYLWIKWSPPTLIDLKTGWFTLLYEIRLKPEKAAEWEIHFAGQQTEFKILSLHPGQKYLVQVRCKPDHGYWSAWSPATFIQIPSDFTMND

Approximately 32-38 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, 5-8% trehalose, 0.01% Tween 80, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.