SIRT2 Protein, Human (His)

SIRT2 Protein, Human (His) is the recombinant human-derived SIRT2, expressed by E. coli , with C-6*His labeled tag. ,

SIRT2 is an NAD-dependent protein deacetylase that targets histones (e.g., H4K16ac, H3K56ac), α-tubulin (e.g., Lys-40), and key transcription factors (e.g., FOXO1, FOXO3, HIF1A, RELA, p53/TP53). It regulates diverse processes including cell cycle control (e.g., G2/M transition, APC/C activation), genomic stability, microtubule dynamics, metabolic pathways (e.g., PPP, gluconeogenesis), autophagy, and epigenetic modifications (e.g., H4K20me1 deposition). During bacterial infection (e.g., Listeria monocytogenes) or oxidative stress, SIRT2 modulates transcriptional activity and stress responses via deacetylation. It also exhibits demyristoylation (e.g., ARF6) and depalmitoylation (e.g., KRAS) activities, influencing membrane localization. by similar: SIRT2 promotes FOXO3 ubiquitination and degradation through deacetylation.

Measured by its ability to remove the acetyl group from a fluorogenic peptide 125 µM substrate Ac-Arg-Gly-Lys(Ac)-AMC in a coupled assay. That incubate at room temperaturein mode for 45 minutes. The specific activity is 15.62 pmol/min/µg.

Human

E. coli

C-6*His

Q8IXJ6-1 (Q13-Q389)

22933

Partial

QAGKVQEAQDSDSDSEGGAAGGEADMDFLRNLFSQTLSLGSQKERLLDELTLEGVARYMQSERCRRVICLVGAGISTSAGIPDFRSPSTGLYDNLEKYHLPYPEAIFEISYFKKHPEPFFALAKELYPGQFKPTICHYFMRLLKDKGLLLRCYTQNIDTLERIAGLEQEDLVEAHGTFYTSHCVSASCRHEYPLSWMKEKIFSEVTPKCEDCQSLVKPDIVFFGESLPARFFSCMQSDFLKVDLLLVMGTSLQVQPFASLISKAPLSTPRLLINKEKAGQSDPFLGMIMGLGGGMDFDSKKAYRDVAWLGECDQGCLALAELLGWKKELEDLVRREHASIDAQSGAGVPNPSTSASPKKSPPPAKDEARTTEREKPQ

Approximately 42 kDa,based on SDS-PAGE under reducing conditions.

• 
  ≥ 90%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.1 mM KCl, and 20% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.