TIE-2 Protein, Rhesus Macaque (HEK293, His)

TIE-2 Protein, a tyrosine-protein kinase, acts as a cell-surface receptor for ANGPT1, ANGPT2, and ANGPT4, exerting comprehensive control over angiogenesis, endothelial cell behavior, and vascular stability. It regulates diverse processes, including endothelial cell survival, proliferation, migration, adhesion, and actin cytoskeleton reorganization, while also playing a crucial role in maintaining vascular quiescence and preventing the leakage of pro-inflammatory plasma proteins and leukocytes from blood vessels. TIE-2 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived TIE-2 protein, expressed by HEK293 , with C-His labeled tag.

TIE-2, a tyrosine-protein kinase, acts as a cell-surface receptor for ANGPT1, ANGPT2, and ANGPT4, exerting comprehensive control over angiogenesis, endothelial cell behavior, and vascular stability. It regulates diverse processes, including endothelial cell survival, proliferation, migration, adhesion, and actin cytoskeleton reorganization, while also playing a crucial role in maintaining vascular quiescence and preventing the leakage of pro-inflammatory plasma proteins and leukocytes from blood vessels, resulting in anti-inflammatory effects. Essential for normal angiogenesis during embryonic development and post-natal hematopoiesis, TIE-2 exhibits context-dependent angiogenic activation or inhibition after birth. In quiescent vessels, ANGPT1, oligomerizing, recruits TIE-2 to cell-cell contacts, activating phosphatidylinositol 3-kinase and AKT1 signaling cascades, promoting vascular stability. Conversely, in migrating endothelial cells lacking cell-cell adhesions, ANGPT1 recruits TIE-2 to extracellular matrix contacts, stimulating sprouting angiogenesis through focal adhesion complex formation and activation of downstream kinases. ANGPT1 signaling induces receptor dimerization and autophosphorylation, providing binding sites for scaffold proteins and effectors. Modulation by ANGPT2, TIE1 heterodimer formation, and proteolytic processing into a soluble extracellular domain contribute to the intricate regulation of TIE-2 signaling, with the soluble domain acting as a decoy receptor for angiopoietins. TIE-2 phosphorylates DOK2, GRB7, GRB14, PIK3R1, SHC1, and TIE1^[1][2][3][4].

1.This product does not contain protein kinase domain.
2.Immobilized Rhesus Macaque TIE-2 at 1 μg/mL (100 μL/well) can bind Biotinylated Human Angiopoietin-2. The ED₅₀ for this effect is 11.97 ng/mL.

Rhesus Macaque

HEK293

C-His

XP_001105270 (A23-K745)

707455  [NCBI]

Partial

AMDLILINSLPLVSDAETSLTCIASGWHPHEPITIGRDFEALMNQHQDPLEVTQDVTREWAKKVVWKREKASKINGAYFCEGRVRGEAIRIRTMKMRQQASFLPATLTMTVDKGDNVNISFKKVLIKEEDAVIYKNGSFIHSVPRHEVPDILEVHLPHAQPQDAGVYSARYIGGNLFTSAFTRLIVRRCEAQKWGPECNRLCTVCVNNGVCHEDTGECICPPGFMGRTCEKACEQHTFGRTCKERCSGQDGCKSYVFCLPDPYGCSCATGWKGLQCNEACHHGFYGPDCKLRCSCSNGETCDRFQGCLCSPGRQGLQCEREGIPRMTPKIVDLPDHIEVNSGKFNPICKASGWPLPTNEEMTLVKPDGTVLHPKDFNHTDHFSVAIFTIHRILPPDSGVWVCSANTVAGMVEKPFNISVKVLPKPLNAPNVIDTGHNFAVINISSEPYFGDGPIKSKKLLYKPVNHYEAWRHIQVTNEIVTLNHLEPRTEYELCVQLVRRGEGGEGHPGPVRRFTTASIGLPPPRGLNLLPKSQTTLNLTWQPIFPSSEDDFYVEVERRSVQKSDQQNIKVPGNLTSVLLNNLHPREQYVVRARVNTKAQGEWSEDLTAWTLSDILPPQPENIKISNITHSSAVISWTILDGYSISSITIRYKVQGKNEDQHIDVKIKNATITQYQLKGLEPETAYQVDIFAENNIGSSNPAFSHELVTLPESEAPADLGGGK

Approximately 115 kDa, based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.