TRAF2 Protein, Human (sf9, GST)
TRAF2 Protein, Human (sf9, GST) is the recombinant human-derived TRAF2, expressed by Sf9 insect cells , with GST labeled tag. ,
- Species: Human
- Source: Sf9 insect cells
-
Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
TRAF2 Protein, Human (sf9, GST) is the recombinant human-derived TRAF2, expressed by Sf9 insect cells , with GST labeled tag. ,
Background
TRAF2 is an E3 ubiquitin-protein ligase that plays a central role in regulating NF-kappa-B and JNK activation, thereby influencing cell survival and apoptosis. It catalyzes 'Lys-63'-linked ubiquitination of target proteins such as BIRC3, IKBKE, MLST8, RIPK1, and TICAM1, and serves as an essential component of multiple E3 ubiquitin-protein ligase complexes, facilitating ubiquitination by bridging target proteins with other E3 ligases. TRAF2 regulates the protein levels of BIRC2 and BIRC3 by inhibiting their autoubiquitination and subsequent degradation, a process independent of its RING-type zinc finger domain. Additionally, TRAF2 mediates NF-kappa-B activation by EIF2AK2/PKR and, in complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE. In the mTOR pathway, TRAF2 modulates the assembly of mTORC1 and mTORC2 by mediating 'Lys-63'-linked ubiquitination of MLST8, inhibiting mTORC2 formation while promoting mTORC1 assembly. TRAF2 is also required for normal antibody isotype switching from IgM to IgG (by similar).
Technical Parameters
-
Species Human
-
Source Sf9 insect cells
-
Tag GST
-
Accession
Q12933-1 (M1-L501)
-
Gene ID7186
-
Protein Length
Full Length of Isoform-1
-
Synonyms
TRAF2; TNF Receptor-Associated Factor 2; TNF Receptor Associated Factor 2; Tumor Necrosis Factor Type 2 Receptor Associated Protein 3; TRAP3; Tumor Necrosis Factor Type 2 Receptor-Associated Protein 3; RNF117; TNF Receptor-Associated Factor; RING-Type E3
-
AA Sequence
MAAASVTPPGSLELLQPGFSKTLLGTKLEAKYLCSACRNVLRRPFQAQCGHRYCSFCLASILSSGPQNCAACVHEGIYEEGISILESSSAFPDNAARREVESLPAVCPSDGCTWKGTLKEYESCHEGRCPLMLTECPACKGLVRLGEKERHLEHECPERSLSCRHCRAPCCGADVKAHHEVCPKFPLTCDGCGKKKIPREKFQDHVKTCGKCRVPCRFHAIGCLETVEGEKQQEHEVQWLREHLAMLLSSVLEAKPLLGDQSHAGSELLQRCESLEKKTATFENIVCVLNREVERVAMTAEACSRQHRLDQDKIEALSSKVQQLERSIGLKDLAMADLEQKVLEMEASTYDGVFIWKISDFARKRQEAVAGRIPAIFSPAFYTSRYGYKMCLRIYLNGDGTGRGTHLSLFFVVMKGPNDALLRWPFNQKVTLMLLDQNNREHVIDAFRPDVTSSSFQRPVNDMNIASGCPLFCPVSKMEAKNSYVRDDAIFIKAIVDLTGL
-
Molecular Weight
Approximately 89 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
