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UPP1 Protein, Human (His)

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Based on 1 publication(s) in Google Scholar

UPP1 belongs to the family of pentosyltransferases. UPP1 is expressed in the cytosol of most tissues and is important for the homeostatic regulation of intracellular and plasma uridine concentrations. UPP1 elevates its expression levels in many tumors. UPP1 Protein, Human (His) is a recombinant human UPP1 protein expressed by E. coli, with an N-6*His tag, consisting of 173 amino acids (M1-A173).

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for UPP1 Protein, Human (His)
  • UPP1 Protein, Human (His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
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Biological Activity

Description

UPP1 belongs to the family of pentosyltransferases. UPP1 is expressed in the cytosol of most tissues and is important for the homeostatic regulation of intracellular and plasma uridine concentrations. UPP1 elevates its expression levels in many tumors. UPP1 Protein, Human (His) is a recombinant human UPP1 protein expressed by E. coli, with an N-6*His tag, consisting of 173 amino acids (M1-A173).

Background

Uridinephosphorylase 1 (UPP1) is a member of the family of pentosyltransferase. UPP1 plays an important role in the pyrimidine salvage pathway through its catalysis of the reversible phosphorolysis of uridine to uracil. UPP1 is important for the homeostatic regulation of intracellular and plasma uridine concentratios. UPP1 is expressed in a variety of tissues including liver kidney and colon reflecting its diverse physiological roles. UPP 1 has been linked to cancer and metabolic disorders. The expression levels and the enzymatic activity of UPP1 are higher in human solid tumors than in adjacent normal tissues. The high level of UPP1 expression makes it a potential prognosticfactor for some cancers, such as oral squamous cell carcinoma[1].

In Vitro

UPP1 Protein, Human (His) can directly binds with recombinant AKT in vitro, as showed in the GST pull-down analysis[1].

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-6*His
  • Accession

    Q86Y75 (M1-A173)

  • Gene ID
    7378  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • UPP1 (M1-A173)
      Accession # Q86Y75
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    UPP1; Uridine Phosphorylase 1; UP; UDRPASE; UPASE; UPP; UrdPase 1; UPase 1; Uridine Phosphorylase

  • AA Sequence

    MQRKLKVTSLEPGTVVITEQAVDTCFKAEFEQIVLGKRVIRKTDLNKKLVQELLLCSAELSEFTTVVGNTMCTLDFYEGQGRLDGALCSYTEKDKQAYLEAAYAAGVRNIEMESSVFAAMCSACGLQAAVVCVTLLNRLEGDQISSPRNVLSEYQQRPQRLVSYFIKKKLSKA

  • Predicted Molecular Mass

    20 kDa

  • Molecular Weight

    Approximately 20 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 200 mM NaCl, 1 mM DTT, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

References

[1]. Du W, et al. UPP1 enhances bladder cancer progression and gemcitabine resistance through AKT. Int J Biol Sci. 2024 Jan 27;20(4):1389-1409. [Content Brief]

Calculators

Reconstitution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
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MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • UPP1 Protein, Human (His) Product Image
    1/16
  • Experimental Validation Results for UPP1 Protein, Human (His)
  • UPP1 Protein, Human (His) Product Image