RW3 

RW3 (MP196) is a small cationic antimicrobial hexapeptide. RW3 targets the bacterial cytoplasmic membrane and inhibits cellular respiration and cell wall synthesis. RW3 exhibits high bioactivity against Gram-positive bacteria such as Bacillus subtilis 168 (MIC of 2 µg/mL). RW3 causes shrinking of murine erythrocytes. RW3 can be used in antimicrobial and antifungal research^[1].

RW3 (16 h) shows decent activity against Gram-positive bacteria like Bacillus subtilis 168 (MIC of 2 µg/mL) and some MRSA/VISA strains, but weak activity against Gram-negative bacteria such as Pseudomonas aeruginosa PA01 (MIC of >64 µg/mL)^[1].
RW3 (0.09-200 µg/mL; 72 h) shows no significant cytotoxicity against NRK-52E rat kidney epithelial cells and CCRF-CEM human T-cell lymphoblast cells^[1].
RW3 (10 ng-500 µg/mL; 1-24 h) does not induce NF-κB activation in RT4 bladder epithelial cells^[1].
RW3 (250-500 µg/mL) causes shrinking of murine erythrocytes at 250 µg/mL and severe morphological changes plus lysis at 500 µg/mL, with 14% and 23% hemolysis respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RW3 (5-25 mg/kg; i.v.) causes excitement and indisposition (recovered within 25 min) at 5-10 mg/kg, and hind limb paralysis plus death within minutes at 25 mg/kg in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1044.22

C₅₁H₆₉N₁₉O₆

359632-11-4

Solid

White to off-white

Arg-Trp-Arg-Trp-Arg-Trp-NH2

RWRWRW-NH2

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Wenzel M, et al. Towards profiles of resistance development and toxicity for the small cationic hexapeptide RWRWRW-NH2[J]. Frontiers in Cell and Developmental Biology, 2016, 4: 86.  [Content Brief]