Saprorthoquinone
Saprorthoquinone (NSC 648341) is a diterpenoid that can be found in salvia atropatana. Saprorthoquinone shows cytotoxicity for PC3 cells.
For research use only. We do not sell to patients.
- CAS No.: 102607-41-0
- Formula: C20H24O2
- Molecular Weight:296.40
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HL-60 | IC50 |
2.36 μM
Compound: 1
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In vitro cytotoxic activity against HL-60 human leukemia cell line was determined by MTT assay (microculture terazolium colorimetric)
In vitro cytotoxic activity against HL-60 human leukemia cell line was determined by MTT assay (microculture terazolium colorimetric)
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[PMID: 10509925] |
| P388 | IC50 |
1.95 μM
Compound: 1
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In vitro cytotoxic activity against P388 mouse leukemia cell line by MTT assay (microculture terazolium colorimetric)
In vitro cytotoxic activity against P388 mouse leukemia cell line by MTT assay (microculture terazolium colorimetric)
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[PMID: 10509925] |
| SGC-7901 | IC50 |
1.37 μM
Compound: 1
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In vitro cytotoxic activity against SGC-7901 stomach cancer solid tumor cell line by MTT assay (microculture terazolium colorimetric)
In vitro cytotoxic activity against SGC-7901 stomach cancer solid tumor cell line by MTT assay (microculture terazolium colorimetric)
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[PMID: 10509925] |
| SPC-A4 | IC50 |
2.75 μM
Compound: 1
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In vitro cytotoxic activity against SPC-A4 lung cancer solid tumor cell line by MTT assay (microculture terazolium colorimetric)
In vitro cytotoxic activity against SPC-A4 lung cancer solid tumor cell line by MTT assay (microculture terazolium colorimetric)
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[PMID: 10509925] |
Chemical Information
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CAS No. 102607-41-0
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Molecular Weight 296.40
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Formula C20H24O2
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SMILES
C/C(C)=C\CCC(C(C)=CC=C1C=C(C(C)C)C2=O)=C1C2=O
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Synonyms
NSC 648341
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Saprorthoquinone Related Classifications
HY-N10687 Related Classifications
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