SARS-CoV-2-IN-60

Cat. No.: HY-155280 Purity: 99.22%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

SARS-CoV-2-IN-60 (compound 5a) is an S-adenosylmethionine (SAM)-competitive and irreversible SARS-CoV-2 nsp16-nsp10 methyltransferase activity inhibitor with an IC₅₀ of 9 μM and a K_i of 26 μM. SARS-CoV-2-IN-60 can specifically occupy a newly identified pocket adjacent to the SAM-binding site on nsp16. SARS-CoV-2-IN-60 has the potential for pan-coronavirus therapeutics.

For research use only. We do not sell to patients.

SARS-CoV-2-IN-60 Chemical Structure

CAS No. : 331647-89-3

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Description

Molecular Weight

335.11

Formula

C₁₃H₇Cl₂F₃N₂O

CAS No.

331647-89-3

Appearance

Solid

Color

Light yellow to yellow

SMILES

ClC1=CC=C(/C=C/C2=NC(O)=NC(C(F)(F)F)=C2)C(Cl)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (298.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9841 mL	14.9205 mL	29.8410 mL
5 mM	0.5968 mL	2.9841 mL	5.9682 mL
10 mM	0.2984 mL	1.4920 mL	2.9841 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.46 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.22%

Select Batch:

Data Sheet (270 KB) SDS (252 KB)

COA (246 KB) HNMR (140 KB) RP-HPLC (167 KB) LCMS (106 KB)

Handling Instructions (2659 KB)

References

[1]. Nicole L Inniss, et al. Discovery of a Druggable, Cryptic Pocket in SARS-CoV-2 nsp16 Using Allosteric Inhibitors. ACS Infect Dis. 2023 Oct 13;9(10):1918-1931. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9841 mL	14.9205 mL	29.8409 mL	74.6024 mL
	5 mM	0.5968 mL	2.9841 mL	5.9682 mL	14.9205 mL
	10 mM	0.2984 mL	1.4920 mL	2.9841 mL	7.4602 mL
	15 mM	0.1989 mL	0.9947 mL	1.9894 mL	4.9735 mL
	20 mM	0.1492 mL	0.7460 mL	1.4920 mL	3.7301 mL
	25 mM	0.1194 mL	0.5968 mL	1.1936 mL	2.9841 mL
	30 mM	0.0995 mL	0.4973 mL	0.9947 mL	2.4867 mL
	40 mM	0.0746 mL	0.3730 mL	0.7460 mL	1.8651 mL
	50 mM	0.0597 mL	0.2984 mL	0.5968 mL	1.4920 mL
	60 mM	0.0497 mL	0.2487 mL	0.4973 mL	1.2434 mL
	80 mM	0.0373 mL	0.1865 mL	0.3730 mL	0.9325 mL
	100 mM	0.0298 mL	0.1492 mL	0.2984 mL	0.7460 mL

SARS-CoV-2-IN-60 Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SARS-CoV-2-IN-60331647-89-3SARS-CoVSARS coronavirusS-adenosylmethionineSAMcompetitiveSARS-CoV-2nsp16-nsp10methyltransferasensp16coronavirusInhibitorinhibitorinhibit

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SARS-CoV-2-IN-60

SARS-CoV-2-IN-60 Related Antibodies

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