Scoulerine hydrochloride
Scoulerine ((-)-Scoulerine; Discretamine) hydrochloride is a multi-target inhibitor with anti-tumor and antioxidant activities. Scoulerine hydrochloride mainly targets the PI3K/Akt/mTOR signaling axis and α1D-adrenergic receptor, disrupts microtubule structure, and induces cell cycle arrest and apoptosis. Scoulerine hydrochloride effectively inhibits mitochondrial dehydrogenase activity, targets GABA receptors and BACE1, and suppresses the proliferation, migration, invasion, epithelial-mesenchymal transition and stem cell properties of cancer cells. Scoulerine hydrochloride also exhibits multiple pharmacological activities including anti-Plasmodium falciparum, antibacterial, antiemetic and antitussive effects, and regulates endoplasmic reticulum stress and mitochondrial function (modulates Bax, Bcl-2 and cytochrome c). Scoulerine hydrochloride is applicable to research related to leukemia, ovarian cancer, and colorectal cancer.
For research use only. We do not sell to patients.
- CAS No.: 51486-68-1
- Formula: C19H22ClNO4
- Molecular Weight:363.84
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
All Adrenergic Receptor Isoforms
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Biological Activity
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mTOR |
Bak |
BACE1 |
Alpha-1D adrenergic receptor |
Scoulerine (1-50 μM; 72 h) exhibits antiproliferative activity against human lung cancer A549 cells, ovarian cancer A2780 cells, breast cancer SK-BR-3 cells, and MCF-7 cells. Low concentrations (1 μM, 5 μM) exert little effect or cause slight delay on cell proliferation, while high concentrations (10 μM, 20 μM, 50 μM) significantly inhibit cell proliferation[2].
Scoulerine (2.5-20 μM; 24 h) induces apoptosis in Jurkat and MOLT-4 leukemia cells, with dose-dependent increases in both early and late apoptosis rates, and DNA fragmentation can be detected via TUNEL assay[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Jurkat, MOLT-4, Raji, HL-60, U-937, HEL 92.1.7, A549, A2780, SK-BR-3, MCF-7
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Concentration:1 μM, 5 μM, 10 μM, 15 μM, 20 μM, 50 μM
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Incubation Time:24 h, 48 h, 72 h
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Result:Reduced the viability and proliferation of leukemic cells in a dose-dependent manner within 24 h, and the reduction was more pronounced at 48 h.
For adherent cell lines, low concentrations (1 μM, 5 μM) had no significant effect or slightly delayed proliferation, while high concentrations (10 μM, 20 μM, 50 μM) exerted a potent antiproliferative effect over 72 h.
The IC50 values of scoulerine against human leukemic cells ranged from 2.7 to 6.5 μM as determined by XTT assay.
IC50 values: 2.7 ± 0.1 μM (Jurkat), 4.2 ± 0.2 μM (HL-60), 4.7 ± 0.2 μM (MOLT-4), 4.8 ± 0.3 μM (U-937), 6.3 ± 0.8 μM (HEL 92.1.7), 6.5 ± 0.7 μM (Raji).
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Cell Line:A549
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Concentration:5 μM, 10 μM, 20 μM
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Incubation Time:24 h
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Result:Disrupted the microtubule structure at 10 μM and 20 μM, leading to dense aggregation of microtubules around cell nuclei and reduced density at the cell periphery.
At 5 μM, the tubulin network remained almost intact similar to the control cells.
Chemical Information
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CAS No. 51486-68-1
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Molecular Weight 363.84
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Formula C19H22ClNO4
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SMILES
OC1=C(OC)C=C(CCN2CC3=C(O)C(OC)=CC=C3C[C@]24[H])C4=C1.Cl
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Synonyms
(-)-Scoulerine hydrochloride; Discretamine hydrochloride
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Structure Classification
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Wang F, et al. Scoulerine promotes cytotoxicity and attenuates stemness in ovarian cancer by targeting PI3K/AKT/mTOR axis. Acta Pharm. 2023;73(3):475-488. Published 2023 Sep 14. [Content Brief]
[2]. Habartova K, et al. Scoulerine affects microtubule structure, inhibits proliferation, arrests cell cycle and thus culminates in the apoptotic death of cancer cells. Sci Rep. 2018;8(1):4829. Published 2018 Mar 19. [Content Brief]
[3]. Banerjee A, et al. Increased reactive oxygen species levels cause ER stress and cytotoxicity in andrographolide treated colon cancer cells. Oncotarget. 2017 Apr 18;8(16):26142-26153. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Scoulerine hydrochloride Related Classifications
Cat. No.: HY-N1255A
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- Scoulerine
- 51486-68-1
- (-)-Scoulerine
- Discretamine
- Apoptosis
- PI3K
- Akt
- mTOR
- Adrenergic Receptor
- GABA Receptor
- Beta-secretase
- Bcl-2 Family
- PI3K/Akt/mTOR axis
- epithelial-mesenchymal transition
- colorectal cancer cells
- α1D-adrenoreceptor
- BACE1
- GABA receptors
- ovarian cancer mouse models
- Plasmodium falciparum
- leukemic cells
- ovarian cancer xenograft mouse models
- Inhibitor
- inhibitor
- inhibit