Art. -Nr.: HY-N1255

Scoulerine

(Synonyms: (-)-Scoulerine; Discretamine)

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Scoulerine ((-)-Scoulerine; Discretamine) hydrochloride is a multi-target inhibitor with anti-tumor and antioxidant activities. Scoulerine mainly targets the PI3K/Akt/mTOR signaling axis and α1D-adrenergic receptor, disrupts microtubule structure, and induces cell cycle arrest and apoptosis. Scoulerine effectively inhibits mitochondrial dehydrogenase activity, targets GABA receptors and BACE1, and suppresses the proliferation, migration, invasion, epithelial-mesenchymal transition and stem cell properties of cancer cells. Scoulerine also exhibits multiple pharmacological activities including anti-Plasmodium falciparum, antibacterial, antiemetic and antitussive effects, and regulates endoplasmic reticulum stress and mitochondrial function (modulates Bax, Bcl-2 and cytochrome c). Scoulerine is applicable to research related to leukemia, ovarian cancer, and colorectal cancer.

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Reinheit: 99.99%
CAS. Nr.: 6451-73-6
Formel: C₁₉H₂₁NO₄
Molecular Weight:327.37

Speicherung:

-20°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Scoulerine Chemical Structure

CAS. Nr.: 6451-73-6

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

BACE1

Alle Bcl-2 Family Isoform-spezifische Produkte anzeigen :

Alle mTOR Isoform-spezifische Produkte anzeigen :

Alle PI3K Isoform-spezifische Produkte anzeigen :

Alle Adrenergic Receptor Isoform-spezifische Produkte anzeigen :

Alle Beta-secretase Isoform-spezifische Produkte anzeigen :

BACE1
BACE2

Alle Akt Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	26.32 μM Compound: (-)-scoulerine	Cytotoxicity against human A549 cells assessed as cell viability measured after 72 hrs by MTT assay Cytotoxicity against human A549 cells assessed as cell viability measured after 72 hrs by MTT assay	[PMID: 34406008]
GES1	IC₅₀	95.3 μM Compound: 4	Antiproliferative activity against human GES1 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human GES1 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 35286954]
HeLa	IC₅₀	6.737 μM Compound: (-)-scoulerine	Cytotoxicity against human HeLa cells assessed as cell viability measured after 72 hrs by MTT assay Cytotoxicity against human HeLa cells assessed as cell viability measured after 72 hrs by MTT assay	[PMID: 34406008]
HGC-27	IC₅₀	6.1 μM Compound: 4	Antiproliferative activity against human HGC-27 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human HGC-27 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 35286954]
HRPE	EC₅₀	3.545 μM Compound: (-)-scoulerine	Induction of mitotic arrest in HRPE Cells overexpressing MYC measured for 24 hrs by acridine orange staining based fluorescence microscopic analysis Induction of mitotic arrest in HRPE Cells overexpressing MYC measured for 24 hrs by acridine orange staining based fluorescence microscopic analysis	[PMID: 34406008]
HRPE	EC₅₀	3.595 μM Compound: (-)-scoulerine	Induction of mitotic arrest in HRPE Cells overexpressing MYC assessed as polyploidy measured for 48 hrs by acridine orange staining based fluorescence microscopic analysis Induction of mitotic arrest in HRPE Cells overexpressing MYC assessed as polyploidy measured for 48 hrs by acridine orange staining based fluorescence microscopic analysis	[PMID: 34406008]
HRPE	IC₅₀	4.065 μM Compound: (-)-scoulerine	Cytotoxicity against HRPE cells overexpressing MYC assessed as cell viability measured after 72 hrs by MTT assay Cytotoxicity against HRPE cells overexpressing MYC assessed as cell viability measured after 72 hrs by MTT assay	[PMID: 34406008]
HRPE	IC₅₀	4.622 μM Compound: (-)-scoulerine	Cytotoxicity against HRPE cells overexpressing MYC/Bcl2 assessed as cell viability measured after 72 hrs by MTT assay Cytotoxicity against HRPE cells overexpressing MYC/Bcl2 assessed as cell viability measured after 72 hrs by MTT assay	[PMID: 34406008]
MDA-MB-231	IC₅₀	7.324 μM Compound: (-)-scoulerine	Cytotoxicity against human MDA-MB-231 cells assessed as cell viability measured after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as cell viability measured after 72 hrs by MTT assay	[PMID: 34406008]
MGC-803	IC₅₀	12.6 μM Compound: 4	Antiproliferative activity against human MGC-803 cells assessed as reduction in cell viability after 24 hrs by MTT assay Antiproliferative activity against human MGC-803 cells assessed as reduction in cell viability after 24 hrs by MTT assay	[PMID: 35286954]
NCI-H23	IC₅₀	2.541 μM Compound: (-)-scoulerine	Cytotoxicity against human NCI-H23 cells harboring MYC assessed as cell viability measured after 72 hrs by MTT assay Cytotoxicity against human NCI-H23 cells harboring MYC assessed as cell viability measured after 72 hrs by MTT assay	[PMID: 34406008]
SW480	IC₅₀	4.237 μM Compound: (-)-scoulerine	Cytotoxicity against human SW480 cells assessed as cell viability measured after 72 hrs by MTT assay Cytotoxicity against human SW480 cells assessed as cell viability measured after 72 hrs by MTT assay	[PMID: 34406008]

In Vitro

Scoulerine (1-50 μM; 72 h) exhibits antiproliferative activity against human lung cancer A549 cells, ovarian cancer A2780 cells, breast cancer SK-BR-3 cells, and MCF-7 cells. Low concentrations (1 μM, 5 μM) exert little effect or cause slight delay on cell proliferation, while high concentrations (10 μM, 20 μM, 50 μM) significantly inhibit cell proliferation^[2].
Scoulerine (2.5-20 μM; 24 h) induces apoptosis in Jurkat and MOLT-4 leukemia cells, with dose-dependent increases in both early and late apoptosis rates, and DNA fragmentation can be detected via TUNEL assay^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

Jurkat and MOLT-4 cells
Concentration:

2.5, 5, 10, 15 and 20 µM
Incubation Time:

24 hours
Result:

Significantly reduced the viability and proliferation of Jurkat and MOLT-4 cells in a dose dependent manner.

Apoptosis Analysis^[1]

Cell Line:

MOLT-4 and Jurkat cells
Concentration:

2.5, 5, 10, 15 and 20 µM
Incubation Time:

24 hours
Result:

Induced MOLT-4 and Jurkat cells apoptosis.

Cell Cycle Analysis^[1]

Cell Line:

Jurkat and MOLT-4 leukemic cells
Concentration:

2.5-20 µM
Incubation Time:

16 hours
Result:

Induced cell cycle arrest at the G2/M transition.

Western Blot Analysis^[1]

Cell Line:

MOLT-4 cells
Concentration:

2.5, 5 µM
Incubation Time:

24 hours
Result:

Showed an upregulation of p53 protein in p53 wild-type MOLT-4 cells.

Chemical Information

CAS. Nr. 6451-73-6
Appearance Solid
Molecular Weight 327.37
Formel C₁₉H₂₁NO₄
Color White to light yellow
SMILES

OC1=C(OC)C=C(CCN2CC3=C(O)C(OC)=CC=C3C[C@]24[H])C4=C1
Synonyms

(-)-Scoulerine; Discretamine
Structure Classification
- Alkaloids
- Isoquinoline Alkaloids
- Phenols
- Polyphenols
Initial Source
- Plants
- other families
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (305.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.0546 mL

15.2732 mL

30.5465 mL

5 mM

0.6109 mL

3.0546 mL

6.1093 mL

10 mM

0.3055 mL

1.5273 mL

3.0546 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (6.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (6.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Wang F, et al. Scoulerine promotes cytotoxicity and attenuates stemness in ovarian cancer by targeting PI3K/AKT/mTOR axis. Acta Pharm. 2023;73(3):475-488. Published 2023 Sep 14. [Content Brief]

[2]. Habartova K, et al. Scoulerine affects microtubule structure, inhibits proliferation, arrests cell cycle and thus culminates in the apoptotic death of cancer cells. Sci Rep. 2018;8(1):4829. Published 2018 Mar 19. [Content Brief]

[3]. Banerjee A, et al. Increased reactive oxygen species levels cause ER stress and cytotoxicity in andrographolide treated colon cancer cells. Oncotarget. 2017 Apr 18;8(16):26142-26153. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.0546 mL	15.2732 mL	30.5465 mL	76.3662 mL
	5 mM	0.6109 mL	3.0546 mL	6.1093 mL	15.2732 mL
	10 mM	0.3055 mL	1.5273 mL	3.0546 mL	7.6366 mL
	15 mM	0.2036 mL	1.0182 mL	2.0364 mL	5.0911 mL
	20 mM	0.1527 mL	0.7637 mL	1.5273 mL	3.8183 mL
	25 mM	0.1222 mL	0.6109 mL	1.2219 mL	3.0546 mL
	30 mM	0.1018 mL	0.5091 mL	1.0182 mL	2.5455 mL
	40 mM	0.0764 mL	0.3818 mL	0.7637 mL	1.9092 mL
	50 mM	0.0611 mL	0.3055 mL	0.6109 mL	1.5273 mL
	60 mM	0.0509 mL	0.2546 mL	0.5091 mL	1.2728 mL
	80 mM	0.0382 mL	0.1909 mL	0.3818 mL	0.9546 mL
	100 mM	0.0305 mL	0.1527 mL	0.3055 mL	0.7637 mL

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Scoulerine

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