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Chondroitinsulfate, one of five classes of glycosaminoglycans, has been widely used in the treatment of osteoarthritis. Chondroitinsulfate reduces inflammation mediators and the apoptotic process and is able to reduce protein production of inflammatory cytokines, iNOS and MMPs.
Chondroitinsulfate sodium (from shark cartilage), one of five classes of glycosaminoglycans, has been widely used in the treatment of osteoarthritis. Chondroitinsulfate reduces inflammation mediators and the apoptotic process and is able to reduce protein production of inflammatory cytokines, iNOS and MMPs .
Hyaluronidase, Ovine testes is an endoglycosidase. Hyaluronidase, Ovine testes specifically degrades Hyaluronic acid (HY-B0633A) and Chondroitinsulfate (HY-B2162) by hydrolyzing β-glycosidic bonds in acidic mucopolysaccharides. Hyaluronidase, Ovine testes disperses follicular cells during fertilization by breaking down the hyaluronic acid-rich cumulus. Hyaluronidase, Ovine testes can be used in the study of fertility-related diseases .
Chondroitinsulfate (from pig) (Chondroitin polysulfate (from pig)) is a sulfated linear polysaccharide extracted from pigs, which belongs to glycosaminoglycans. Chondroitinsulfate (from pig) exerts inconsistent inhibitory effects on the resorptive activity of human osteoclasts. Chondroitinsulfate (from pig) can be used in studies related to osteoarthritis .
Chondroitinsulfate (Chondroitin polysulfate) sodium (from bovine)) is a sulfated linear polysaccharide extracted from bovine sources, belonging to glycosaminoglycans. Chondroitinsulfate sodium (from bovine) inhibits osteoclast-mediated bone resorption and exhibits antioxidant activity, which is negatively correlated with molecular weight. Chondroitinsulfate sodium (from bovine) can be used in studies related to osteoarthritis .
Chondroitinsulfate (from chicken) (Chondroitin polysulfate (from chicken)) is an orally active, sulfated linear polysaccharide extracted from chickens, which belongs to glycosaminoglycans. Chondroitinsulfate (from chicken) reduces the phosphorylation levels of ERK1/2 and p38MAPK, inhibits the expression of inflammatory cytokines and MMP, and downregulates the levels of IL-1β, IL-6, TNF-α and PGE2. Chondroitinsulfate (from chicken) improves motor function, protects cartilage tissue, reverses chondrocyte aggregation, and regulates the structure of intestinal flora. Chondroitinsulfate (from chicken) can be used in research related to osteoarthritis .
Chondroitinase ABC (ChABC) is an enzyme that degrades glycosaminoglycan side-chains of chondroitinsulfate (CS-GAG) from the chondroitinsulfate proteoglycan (CSPG) core protein. Chondroitinase ABC facilitates reinnervation by degrading CS-GAGs around motoneurons. Chondroitinase ABC has the potential for the research of spinal injury .
Chst15-IN-1 is a potent reversible covalent Chst15 inhibitor. Chst15-IN-1 effectively inhibits chondroitinsulfate-E (CS-E) sulfation levels and other closely related glycosaminoglycans (GAG) sulfotransferases. Chst15-IN-1, as a selective sulfotransferase inhibitor, can diminish the inhibitory effects of chondroitinsulfate proteoglycans (CSPGs), and can be used for the stimulation of neuronal repair .
Chondroitinsulfate (from fish), one of five classes of glycosaminoglycans, has been widely used in the treatment of osteoarthritis. Chondroitinsulfate (from fish) reduces inflammation mediators and the apoptotic process and is able to reduce protein production of inflammatory cytokines, iNOS and MMPs.
Chondroitinsulfate (from shark cartilage) is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
PTPσ Inhibitor, ISP can bind to recombinant human PTPs and inhibits PTPσ signaling. PTPσ Inhibitor, ISP can penetrate the membrane and relieves the chondroitinsulfate proteoglycan (CSPG)-mediated axonal sprouting inhibition in spinal cord injury model. PTPσ Inhibitor, ISP enhances remyelination in LPC-induced demyelinated spinal cord. PTPσ Inhibitor, ISP also promotes oligodendrocyte progenitor cells (OPCs) migration, maturation, remyelination, and functional recovery in animal models of Multiple Sclerosis (MS) .
ChondroitinSulfate Methacryloyl (CSMA) is methacrylated chondroitinsulfate and is biocompatible. ChondroitinSulfate Methacryloyl has a higher degree of methacrylation than HAMA (HY-158220), and the degree of methacrylation is closely related to customizable mechanical properties, swelling properties and enzymatic degradability. ChondroitinSulfate Methacryloyl is a versatile biomaterial suitable for biomimetic hydrogel scaffolds and an ideal 3D printing hydrogel ink . ChondroitinSulfate Methacryloyl needs to self-assemble into fibrous hydrogel under the action of photoinitiator LAP (HY-44076), and target bioactive adhesion sites, play an inherent supporting role for tissue cells and biodegradable activity. Application: cell culture, biological 3D printing, tissue engineering, etc.
Cajaninstilbene acid is a potent HCV inhibitor with an IC50 of 3.17 μM. Cajaninstilbene acid inhibits HCV replications via down-regulates a cellular protein chondroitinsulfate N-acetylgalactosaminyltransferase 1 .
Octaarginine acetate is a cell-penetrating peptide and proteasome inhibitor. Octaarginine acetate exhibits mixed-type inhibition against 20S proteasome chymotrypsin-like, caspase-like, and trypsin-like activities, and inhibits 26S proteasome activity with decreased efficiency. Octaarginine acetate induces ubiquitin-conjugated protein accumulation, mediates HSPG-dependent cellular internalization via macropinocytosis, enhances liposomal cargo uptake and gene delivery. Octaarginine acetate can be used for the research of cervix carcinoma, collagen antibody-induced arthritis, and bacterial infections .
Octaarginine is a cell-penetrating peptide and proteasome inhibitor. Octaarginine exhibits mixed-type inhibition against 20S proteasome chymotrypsin-like, caspase-like, and trypsin-like activities, and inhibits 26S proteasome activity with decreased efficiency. Octaarginine induces ubiquitin-conjugated protein accumulation, mediates HSPG-dependent cellular internalization via macropinocytosis, enhances liposomal cargo uptake and gene delivery. Octaarginine can be used for the research of cervix carcinoma, collagen antibody-induced arthritis, and bacterial infections .
plCSA-BP is a Placental CSA-binding peptide. plCSA-BP binds specifically to trophoblasts and not to other cell types in the placenta or to CSA-expressing cells in other tissues. plCSA-BP can guide nanoparticles for the targeted delivery of payloads (such as Indocyanine green (ICG) (HY-D0711) and Methotrexate (MTX) (HY-14519)) to the placenta, promising for placenta-specific drug delivery .
Chondroitinsulfate A disodium is a mucopolysaccharide extracted from animal cartilages such as porcine nasal cartilage, and serves as a major structural component of cartilage. Chondroitinsulfate A disodium is one of the specific receptors for the adhesion of Plasmodium falciparum-infected red blood cells in the microcirculation. Chondroitinsulfate A disodium can be used together with selenium to prepare nanoparticles for protecting cartilage against T‑2 toxin-induced damage. Chondroitinsulfate A disodium is abnormally highly expressed in ameloblastoma, and is particularly enriched in stellate reticulum-like tumor cells. Chondroitinsulfate A disodium can be applied to studies on Plasmodium infection mechanisms, cartilage protection and oral tumors .
Chondroitin disaccharide Δdi-0S sodium is an unsaturated disaccharide produced by the degradation of Chondroitinsulfate (HY-B2162). Chondroitin disaccharide Δdi-0S sodium produces fluorescent pyridylamino derivative with 2-aminopyridine, which can be used in studies about the metabolic pathway of Chondroitinsulfate and related disease mechanisms .
Chondroitinase AC is a hydrolytic enzyme. Chondroitinase AC degrades chondroitinsulfate A and chondroitinsulfate C. Chondroitinase AC inhibits the invasion and proliferation of melanoma cells and endothelial cells, and increases apoptosis. Chondroitinase AC can be used in research on melanoma and other cancers .
Trimethylcolchicinic acid (Desacetylcolchiceine; N-Deacetylcolchiceine) is a colchicine analogue. Trimethylcolchicinic acid promotes the production of chondroitinsulfate in L cells and inhibits the production of dermatan sulfate. Trimethylcolchicinic acid reduces liver fibrosis and cholestasis induced by long-term biliary obstruction in rats. Trimethylcolchicinic acid has potential for use in research on diseases such as liver fibrosis, gout, and cancer .
DSPE-PEG1000-LTLRWVGLMS is a PEG compound which composed of DSPE and a decapeptide (LTLRWVGLMS). The chondroitinsulfate proteoglygan NG2 is a receptor for LTLRWVGLMS. LLRWVGLMS shows the homing of pericytes associated with tumor blood vessels. DSPE-PEG1000-LTLRWVGLMS can be used for drug delivery .
DSPE-PEG2000-LTLRWVGLMS is a PEG compound which composed of DSPE and a decapeptide (LTLRWVGLMS). The chondroitinsulfate proteoglygan NG2 is a receptor for LTLRWVGLMS. LLRWVGLMS shows the homing of pericytes associated with tumor blood vessels. DSPE-PEG2000-LTLRWVGLMS can be used for drug delivery .
Hyaluronidase, Streptomyces hyalurolyticus (EC 3.2.1.35) degrades hyaluronan and has been found to be inappropriately regulated during cancer progression. Hyaluronidase randomly cleaves β-N-acetylhexosamine-[1→4] glycosidic bonds in hyaluronic acid, chondroitin, and chondroitinsulfates.
DSPE-PEG3400-LTLRWVGLMS is a PEG compound which composed of DSPE and a decapeptide (LTLRWVGLMS). The chondroitinsulfate proteoglygan NG2 is a receptor for LTLRWVGLMS. LLRWVGLMS shows the homing of pericytes associated with tumor blood vessels. DSPE-PEG3400-LTLRWVGLMS can be used for drug delivery .
DSPE-PEG5000-LTLRWVGLMS is a PEG compound which composed of DSPE and a decapeptide (LTLRWVGLMS). The chondroitinsulfate proteoglygan NG2 is a receptor for LTLRWVGLMS. LLRWVGLMS shows the homing of pericytes associated with tumor blood vessels. DSPE-PEG5000-LTLRWVGLMS can be used for drug delivery .
IKVAVC is a derivative peptide of IKVAV with an artificially added cysteine (Cys) at its C-terminus. IKVAVC retains all the biological activities of the original IKVAV, mainly acting as a neural adhesion/differentiation signaling peptide, and is equipped with an engineered linker arm that enables covalent conjugation to molecular materials. IKVAV inhibits the migration and activation of fibroblasts, downregulates the TGF-β1 signaling pathway and endoplasmic reticulum stress, and promotes nerve repair. IKVAV regulates the phenotype of macrophages, shifting them from the pro-inflammatory M1 type to the pro-reparative M2 type .
Chondroitinsulfate (from pig) (Chondroitin polysulfate (from pig)) is a sulfated linear polysaccharide extracted from pigs, which belongs to glycosaminoglycans. Chondroitinsulfate (from pig) exerts inconsistent inhibitory effects on the resorptive activity of human osteoclasts. Chondroitinsulfate (from pig) can be used in studies related to osteoarthritis .
Chondroitinsulfate (Chondroitin polysulfate) sodium (from bovine)) is a sulfated linear polysaccharide extracted from bovine sources, belonging to glycosaminoglycans. Chondroitinsulfate sodium (from bovine) inhibits osteoclast-mediated bone resorption and exhibits antioxidant activity, which is negatively correlated with molecular weight. Chondroitinsulfate sodium (from bovine) can be used in studies related to osteoarthritis .
Chondroitinsulfate (from chicken) (Chondroitin polysulfate (from chicken)) is an orally active, sulfated linear polysaccharide extracted from chickens, which belongs to glycosaminoglycans. Chondroitinsulfate (from chicken) reduces the phosphorylation levels of ERK1/2 and p38MAPK, inhibits the expression of inflammatory cytokines and MMP, and downregulates the levels of IL-1β, IL-6, TNF-α and PGE2. Chondroitinsulfate (from chicken) improves motor function, protects cartilage tissue, reverses chondrocyte aggregation, and regulates the structure of intestinal flora. Chondroitinsulfate (from chicken) can be used in research related to osteoarthritis .
Chondroitinsulfate (from fish), one of five classes of glycosaminoglycans, has been widely used in the treatment of osteoarthritis. Chondroitinsulfate (from fish) reduces inflammation mediators and the apoptotic process and is able to reduce protein production of inflammatory cytokines, iNOS and MMPs.
Chondroitinsulfate (from shark cartilage) is a biochemical reagent that can be used as a biological material or organic compound for life science related research.
ChondroitinSulfate Methacryloyl (CSMA) is methacrylated chondroitinsulfate and is biocompatible. ChondroitinSulfate Methacryloyl has a higher degree of methacrylation than HAMA (HY-158220), and the degree of methacrylation is closely related to customizable mechanical properties, swelling properties and enzymatic degradability. ChondroitinSulfate Methacryloyl is a versatile biomaterial suitable for biomimetic hydrogel scaffolds and an ideal 3D printing hydrogel ink . ChondroitinSulfate Methacryloyl needs to self-assemble into fibrous hydrogel under the action of photoinitiator LAP (HY-44076), and target bioactive adhesion sites, play an inherent supporting role for tissue cells and biodegradable activity. Application: cell culture, biological 3D printing, tissue engineering, etc.
DSPE-PEG1000-LTLRWVGLMS is a PEG compound which composed of DSPE and a decapeptide (LTLRWVGLMS). The chondroitinsulfate proteoglygan NG2 is a receptor for LTLRWVGLMS. LLRWVGLMS shows the homing of pericytes associated with tumor blood vessels. DSPE-PEG1000-LTLRWVGLMS can be used for drug delivery .
DSPE-PEG2000-LTLRWVGLMS is a PEG compound which composed of DSPE and a decapeptide (LTLRWVGLMS). The chondroitinsulfate proteoglygan NG2 is a receptor for LTLRWVGLMS. LLRWVGLMS shows the homing of pericytes associated with tumor blood vessels. DSPE-PEG2000-LTLRWVGLMS can be used for drug delivery .
DSPE-PEG3400-LTLRWVGLMS is a PEG compound which composed of DSPE and a decapeptide (LTLRWVGLMS). The chondroitinsulfate proteoglygan NG2 is a receptor for LTLRWVGLMS. LLRWVGLMS shows the homing of pericytes associated with tumor blood vessels. DSPE-PEG3400-LTLRWVGLMS can be used for drug delivery .
DSPE-PEG5000-LTLRWVGLMS is a PEG compound which composed of DSPE and a decapeptide (LTLRWVGLMS). The chondroitinsulfate proteoglygan NG2 is a receptor for LTLRWVGLMS. LLRWVGLMS shows the homing of pericytes associated with tumor blood vessels. DSPE-PEG5000-LTLRWVGLMS can be used for drug delivery .
PTPσ Inhibitor, ISP can bind to recombinant human PTPs and inhibits PTPσ signaling. PTPσ Inhibitor, ISP can penetrate the membrane and relieves the chondroitinsulfate proteoglycan (CSPG)-mediated axonal sprouting inhibition in spinal cord injury model. PTPσ Inhibitor, ISP enhances remyelination in LPC-induced demyelinated spinal cord. PTPσ Inhibitor, ISP also promotes oligodendrocyte progenitor cells (OPCs) migration, maturation, remyelination, and functional recovery in animal models of Multiple Sclerosis (MS) .
Octaarginine acetate is a cell-penetrating peptide and proteasome inhibitor. Octaarginine acetate exhibits mixed-type inhibition against 20S proteasome chymotrypsin-like, caspase-like, and trypsin-like activities, and inhibits 26S proteasome activity with decreased efficiency. Octaarginine acetate induces ubiquitin-conjugated protein accumulation, mediates HSPG-dependent cellular internalization via macropinocytosis, enhances liposomal cargo uptake and gene delivery. Octaarginine acetate can be used for the research of cervix carcinoma, collagen antibody-induced arthritis, and bacterial infections .
Octaarginine is a cell-penetrating peptide and proteasome inhibitor. Octaarginine exhibits mixed-type inhibition against 20S proteasome chymotrypsin-like, caspase-like, and trypsin-like activities, and inhibits 26S proteasome activity with decreased efficiency. Octaarginine induces ubiquitin-conjugated protein accumulation, mediates HSPG-dependent cellular internalization via macropinocytosis, enhances liposomal cargo uptake and gene delivery. Octaarginine can be used for the research of cervix carcinoma, collagen antibody-induced arthritis, and bacterial infections .
plCSA-BP is a Placental CSA-binding peptide. plCSA-BP binds specifically to trophoblasts and not to other cell types in the placenta or to CSA-expressing cells in other tissues. plCSA-BP can guide nanoparticles for the targeted delivery of payloads (such as Indocyanine green (ICG) (HY-D0711) and Methotrexate (MTX) (HY-14519)) to the placenta, promising for placenta-specific drug delivery .
IKVAVC is a derivative peptide of IKVAV with an artificially added cysteine (Cys) at its C-terminus. IKVAVC retains all the biological activities of the original IKVAV, mainly acting as a neural adhesion/differentiation signaling peptide, and is equipped with an engineered linker arm that enables covalent conjugation to molecular materials. IKVAV inhibits the migration and activation of fibroblasts, downregulates the TGF-β1 signaling pathway and endoplasmic reticulum stress, and promotes nerve repair. IKVAV regulates the phenotype of macrophages, shifting them from the pro-inflammatory M1 type to the pro-reparative M2 type .
Chondroitinsulfate, one of five classes of glycosaminoglycans, has been widely used in the treatment of osteoarthritis. Chondroitinsulfate reduces inflammation mediators and the apoptotic process and is able to reduce protein production of inflammatory cytokines, iNOS and MMPs.
Chondroitinsulfate sodium (from shark cartilage), one of five classes of glycosaminoglycans, has been widely used in the treatment of osteoarthritis. Chondroitinsulfate reduces inflammation mediators and the apoptotic process and is able to reduce protein production of inflammatory cytokines, iNOS and MMPs .
Cajaninstilbene acid is a potent HCV inhibitor with an IC50 of 3.17 μM. Cajaninstilbene acid inhibits HCV replications via down-regulates a cellular protein chondroitinsulfate N-acetylgalactosaminyltransferase 1 .
Chondroitinase ABC protein is an endolytic glycosaminoglycan lyase with broad specificity that effectively degrades polysaccharides such as chondroitin, chondroitin 4-sulfate, chondroitin 6-sulfate, dermatan sulfate, and smaller degree of hyaluronic acid. This enzyme activity is facilitated by β-elimination of the 1,4-hexosamine bond, resulting in unsaturated tetra- and disaccharides. Chondroitinase ABC Protein, P. vulgaris (His) is the recombinant Chondroitinase ABC protein, expressed by E. coli , with N-6*His labeled tag.
The PTPRZ1 protein is an important tyrosine phosphatase that negatively regulates oligodendrocyte precursor proliferation in the embryonic spinal cord. It plays a crucial role in guiding the normal differentiation of precursor cells into mature oligodendrocytes and may act as an anti-apoptotic factor. PTPRZ1 Protein, Human (P.pastoris, His) is the recombinant human-derived PTPRZ1 protein, expressed by P. pastoris , with N-His labeled tag.
Versican Isoform V0 is an isotype of versican with its central domain of containing both the GAG-α and GAG-β domains. Versican Isoform V0 protein can be used in research on inflammatory diseases and cancer. Versican Isoform V0 Protein, Human (His, B2M, Myc) is the recombinant human-derived Versican Isoform V0, expressed by E. coli, with N-B2M, C-Myc, N-10*His labeled tag.
The CSPG5 protein is a potential growth and differentiation factor that is actively involved in and critical for neural development. It may induce ERBB3 activation, a key pathway for cell growth and differentiation. CSPG5 Protein, Human (HEK293, His) is the recombinant human-derived CSPG5 protein, expressed by HEK293 , with C-His labeled tag.
CSPG5 protein plays a key role as a growth and differentiation factor in neuritogenesis and may induce ERBB3 activation and participate in signaling pathways related to cell growth and differentiation. Its ability to bind to TNR and possibly TNC suggests interactions with key molecules in cellular processes. CSPG5 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CSPG5 protein, expressed by HEK293 , with C-His labeled tag.
DSPE-PEG1000-LTLRWVGLMS is a PEG compound which composed of DSPE and a decapeptide (LTLRWVGLMS). The chondroitinsulfate proteoglygan NG2 is a receptor for LTLRWVGLMS. LLRWVGLMS shows the homing of pericytes associated with tumor blood vessels. DSPE-PEG1000-LTLRWVGLMS can be used for drug delivery .
DSPE-PEG2000-LTLRWVGLMS is a PEG compound which composed of DSPE and a decapeptide (LTLRWVGLMS). The chondroitinsulfate proteoglygan NG2 is a receptor for LTLRWVGLMS. LLRWVGLMS shows the homing of pericytes associated with tumor blood vessels. DSPE-PEG2000-LTLRWVGLMS can be used for drug delivery .
DSPE-PEG3400-LTLRWVGLMS is a PEG compound which composed of DSPE and a decapeptide (LTLRWVGLMS). The chondroitinsulfate proteoglygan NG2 is a receptor for LTLRWVGLMS. LLRWVGLMS shows the homing of pericytes associated with tumor blood vessels. DSPE-PEG3400-LTLRWVGLMS can be used for drug delivery .
DSPE-PEG5000-LTLRWVGLMS is a PEG compound which composed of DSPE and a decapeptide (LTLRWVGLMS). The chondroitinsulfate proteoglygan NG2 is a receptor for LTLRWVGLMS. LLRWVGLMS shows the homing of pericytes associated with tumor blood vessels. DSPE-PEG5000-LTLRWVGLMS can be used for drug delivery .
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Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
MedchemExpress Validation 03
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
MedchemExpress Validation 04
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
MedchemExpress Validation
Western blot analysis of extracts from THP-1(lane 2(20μg), Jurkat (lane 3(20μg) and NIH3T3(lane 4(20μg) using FOXO1A (HY-P80132) Rabbit mAb. Proteins were transferred
to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was
used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
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