SH-42

Name: SH-42
Brand: MedChemExpress
SKU: HY-143228
Rating: 5.0 (3 reviews)

Cat. No.: HY-143228 Purity: 99.87%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

SH-42 is a potent and selective inhibitor of human Δ²⁴-dehydrocholesterol reductase (DHCR24), with an IC₅₀ of 42 nM. SH-42 can lead to a significant increase in plasma desmosterol levels of mice.

For research use only. We do not sell to patients.

CAS No. : 2143952-36-5

Size	Stock	Quantity
Free Sample (0.1 - 0.2 mg)	Apply Now
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE SH-42

Cell Proliferation/Viability Assay

Cell Migration/Invasion Assay

: SH-42 purchased from MedChemExpress. Usage Cited in: Stem Cells Transl Med. 2024 Nov 18:szae087. [Abstract]; Effects of DHCR24 and SH-42 on cell viability.

: SH-42 purchased from MedChemExpress. Usage Cited in: Stem Cells Transl Med. 2024 Nov 18:szae087. [Abstract]; Effects of DHCR24 and SH-42 on wound healing ability of S12 cells.

: SH-42 purchased from MedChemExpress. Usage Cited in: Stem Cells Transl Med. 2024 Nov 18:szae087. [Abstract]; Effects of DHCR24 and SH-42 on migration ability of S12 cells.

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References
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Description

IC₅₀ & Target

IC50: 42 nM (DHCR24)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	IC₅₀	> 50 μM Compound: 27; SH-42	Cytotoxicity in human HL60 cells by MTT assay Cytotoxicity in human HL60 cells by MTT assay	[PMID: 28964935]

In Vitro

SH-42 is highly selective and active in HL-60 without adverse cytotoxicity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

SH-42 (500 μg, inject, once a day for five days) causes a significant increase in plasma desmosterol levels in mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 mice^[1]
Dosage:	500 μg; daily; 5 days
Administration:	Inject
Result:	Did not cause acute changes in ALT and AST, no severe acute hepatotoxicity was observed, and desmosterol levels were upregulated.

Molecular Weight

402.57

Formula

C₂₅H₃₈O₄

CAS No.

2143952-36-5

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12[C@]3([H])C([C@@]4([H])[C@](CC3)([C@@](CC4)([H])[C@H](C)COC=O)C)=CC[C@@]1([H])C[C@H](CC2)OC(C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (12.42 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4840 mL	12.4202 mL	24.8404 mL
5 mM	0.4968 mL	2.4840 mL	4.9681 mL
10 mM	0.2484 mL	1.2420 mL	2.4840 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 6.6% Cremophor solution (1:1 Ethanol and Cremophor EL) 93.4% PBS
Solubility: 5 mg/mL (12.42 mM); Suspended solution; Need ultrasonic and warming and heat to 60°C
Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.5 mg/mL (1.24 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 3

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.5 mg/mL (1.24 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 4

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 0.5 mg/mL (1.24 mM); Clear solution

This protocol yields a clear solution of ≥ 0.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (5.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline
Solubility: 1.67 mg/mL (4.15 mM); Suspended solution; Need ultrasonic
Protocol 2

Add each solvent one by one: 15% Solutol HS 15 10% Cremophor EL 35% PEG400 40% Water
Solubility: 5 mg/mL (12.42 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.87%

Select Batch:

Data Sheet (273 KB) SDS (251 KB)

COA (247 KB) HNMR (121 KB) RP-HPLC (237 KB)

Handling Instructions (2659 KB)

References

[1]. Müller C, et, al. New chemotype of selective and potent inhibitors of human delta 24-dehydrocholesterol reductase. Eur J Med Chem. 2017 Nov 10;140:305-320. [Content Brief]

[2]. Desirée Heerdegen, et al. Synthesis of Seco-Analogues of the DHCR24 Inhibitor SH-42. Chemistry europe. 05 September 2020

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4840 mL	12.4202 mL	24.8404 mL	62.1010 mL
	5 mM	0.4968 mL	2.4840 mL	4.9681 mL	12.4202 mL
	10 mM	0.2484 mL	1.2420 mL	2.4840 mL	6.2101 mL

SH-42 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SH-422143952-36-5SH42SH 42SARS-CoVSARS coronavirusdehydrocholesterol reductaseDHCR24desmosterolInhibitorinhibitorinhibit

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SH-42

Customer Review

SH-42 Related Antibodies

3 Publications Citing Use of MCE SH-42

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Complete Stock Solution Preparation Table

SH-42 Related Classifications

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