Sulfamethoxazole 1000 µg/mL in methanol
Based on 24 publication(s) in Google Scholar
Sulfamethoxazole (Ro 4-2130) 1000 μg/mL in methanol is a sulfonamide antibiotic with a widespread antibacterial activity. Sulfamethoxazole 1000 μg/mL in methanol inhibits bacterial folate metabolism by competing with 4-Aminobenzoic acid (HY-B1008) (PABA) to act on dihydropteroate synthetase and dihydropteroate reductase. Sulfamethoxazole 1000 μg/mL in methanol can be used for the study of urinary tract infections (UTIs), prostatitis, and bronchitis.
For research use only. We do not sell to patients.
- CAS No.: 723-46-6
- Formula: C10H11N3O3S
- Molecular Weight:253.28
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications Citing Use of MedChemExpress (MCE) Sulfamethoxazole 1000 µg/mL in methanol
More- Signal Transduct Target Ther. 2023 Sep 18;8(1):353. [Abstract]
- Autophagy. 2023 Oct;19(10):2702-2718. [Abstract]
- Water Res. 2023 Jul 15:240:120110. [Abstract]
- J Exp Med. 2026 Mar 2;223(3):e20241287. [Abstract]
- ACS Environ Au. 2025 Aug 5;5(6):573-582. [Abstract]
- Food Chem X. 2026 Feb 8:34:103646. [Abstract]
- Clin Chem. 2019 Dec;65(12):1522-1531. [Abstract]
- Emerg Contam. 2026 Feb 23.
- Anal Chem. 2025 Jun 3;97(21):11099-11109. [Abstract]
- Pharmaceutics. 2025 Mar 26;17(4):423. [Abstract]
- Virulence. 2026 Dec 31;17(1):2646808. [Abstract]
- Ecotoxicol Environ Saf. 2023 Mar 15:253:114711. [Abstract]
- Antibiotics (Basel). 2026 Mar 10;15(3):283. [Abstract]
- Clean Technol Environ Policy. 2025 May 22.
- Bioresour Technol Rep. 2023 Dec, 24, 101619.
- Microorganisms. 2025 Apr 18;13(4):938. [Abstract]
- Water. 2025 Jun 6.
- Infect Drug Resist. 2026 May 8;19.
- Eur J Clin Microbiol Infect Dis. 2025 Sep 29. [Abstract]
- PLoS One. 2026 Jul 8;21(7):e0352997.
- Res Sq. 2026 Mar 9.
- bioRxiv. 2025 January 19.
- bioRxiv. 2024 May 10.
- Chemosphere. 2019 Jun:225:378-387. [Abstract]
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
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Bio/Physico-chemical Assay
All Antibiotic Isoforms
More
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A7R5 | IC50 |
333 μM
Compound: 31
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Displacement of 125I-ET1 from ETA receptor in rat A7R5 cells
Displacement of 125I-ET1 from ETA receptor in rat A7R5 cells
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[PMID: 27321813] |
| A7R5 | IC50 |
333 μM
Compound: 5a
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Displacement of [125 I] ET-1 from rat aortic smooth muscle A7r5 cells transfected with Endothelin A receptor
Displacement of [125 I] ET-1 from rat aortic smooth muscle A7r5 cells transfected with Endothelin A receptor
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[PMID: 10969989] |
| HepG2 | IC50 |
2622 μM
Compound: 1a, SMX
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Cytotoxicity against human HepG2 cells after 24 hrs by MTS assay
Cytotoxicity against human HepG2 cells after 24 hrs by MTS assay
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[PMID: 22365879] |
| MT4 | ED50 |
>79 μM
Compound: A047
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Effective dose of compound required to inhibit replication of human immunodeficiency virus type 1 in MT-4 cells
Effective dose of compound required to inhibit replication of human immunodeficiency virus type 1 in MT-4 cells
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[PMID: 15537350] |
In Vitro
Sulfamethoxazole (0.01-1.28 mg/mL; 120 h) inhibits P. lutzii mitochondrial activity[1].
Sulfamethoxazole (0.01 mg/mL; 1-2 h) acts in P. lutzii as a competitor for amino acid, nucleic acids and folate cofactor biosynthesis, disrupting mitochondrial functions[1].
Sulfamethoxazole at different concentrations, by using the whole-cell patch clamp recording method, inhibits the KATP channel currents in mouse pancreatic β cells in a dose-dependent manner, and the IC50 is 0.46 mM[3].
Sulfamethoxazole at different concentrations, through the static incubation assay of islets, stimulates insulin secretion in mouse islets. It can stimulate insulin secretion at a low concentration (0.1 mM) under high-glucose (10 mM) conditions and only at a high concentration (10 mM) under low-glucose (2 mM) conditions[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:P. lutzii yeast cells
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Concentration:1.28 mg/mL, 0.64 mg/mL, 0.32 mg/mL, 0.16 mg/mL, 0.08 mg/mL, 0.04 mg/mL, 0.02 mg/mL, and 0.01 mg/mL
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Incubation Time:120 h
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Result:Produced a marked dose-dependent adverse effect on P. lutzii.
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Cell Line:P. lutzii yeast cells
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Concentration:0.01 mg/mL
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Incubation Time:1 or 2 h
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Result:The up-regulated genes included those for HSP70, HSP60, and HSP30.
The down-regulated genes included betaine aldehyde dehydrogenase (BADH), carnitine/acyl carnitine carrier (ACUH), guanine nucleotide binding protein α-1 subunit (GPA2), C6 transcription factor (CTF1B) and pleckstrin homology domain (PH).
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 723-46-6
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Appearance Solid
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Molecular Weight 253.28
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Formula C10H11N3O3S
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Color White to off-white
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SMILES
O=S(NC1=NOC(C)=C1)(C2=CC=C(C=C2)N)=O
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Synonyms
Ro 4-2130 1000 µg/mL in methanol
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (24)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
NAD+ exhaustion by CD38 upregulation contributes to blood pressure elevation and vascular damage in hypertension. [Abstract]2023 Sep 18;8(1):353. PMID: 37718359 -
Autophagy
2023 Oct;19(10):2702-2718. PMID: 37312409 -
Water Res
Quantifying community-wide antibiotic usage via urban water fingerprinting: Focus on contrasting resource settings in South Africa. [Abstract]2023 Jul 15:240:120110. PMID: 37247434 -
J Exp Med
2026 Mar 2;223(3):e20241287. PMID: 41400657 -
ACS Environ Au
Machine Learning-Assisted Recognition of Environmental Sulfur-Containing Chemicals in Nontargeted Mass Spectrometry Analysis of Inadequate Mass Resolution. [Abstract]2025 Aug 5;5(6):573-582. PMID: 41277996 -
Food Chem X
Machine learning prioritization of antibiotic residues in aquatic foods reveals exposure-driven genotoxic risk mediated by BCL2. [Abstract]2026 Feb 8:34:103646. PMID: 41756597 -
Clin Chem
Discovering Cross-Reactivity in Urine Drug Screening Immunoassays through Large-Scale Analysis of Electronic Health Records. [Abstract]2019 Dec;65(12):1522-1531. PMID: 31578215 -
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Anal Chem
Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]2025 Jun 3;97(21):11099-11109. PMID: 40401576 -
Pharmaceutics
Plasma Protein Binding, Biostability, Metabolite Profiling, and CYP450 Phenotype of TPB15 Across Different Species: A Novel Smoothened Inhibitor for TNBC Therapy. [Abstract]2025 Mar 26;17(4):423. PMID: 40284418 -
Virulence
Antibacterial efficacy and mechanism of the novel antimicrobial peptide lachnospirin-1 against Acinetobacter baumannii. [Abstract]2026 Dec 31;17(1):2646808. PMID: 41838520 -
Ecotoxicol Environ Saf
Microbial profiles and immune responses in seahorse gut and brood pouch under chronic exposure to environmental antibiotics. [Abstract]2023 Mar 15:253:114711. PMID: 36868035
Sulfamethoxazole 1000 µg/mL in methanol purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2023 Mar 15:253:114711. [Abstract]
Heat map of several key genes involved in immunity, metabolism, and circadian rhythm within the gut of juvenile and adult seahorses treated with Sulfamethoxazole (SMX) (1 μg/L; 4 weeks) was constructed using Tbtools software.
Sulfamethoxazole 1000 µg/mL in methanol purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2023 Mar 15:253:114711. [Abstract]
Sulfamethoxazole (SMX) (1 μg/L; 4 weeks) increased the relative abundance of Proteobacteria, whereas decreased the relative abundance of Actinobacteria and Acidobacteria.
Sulfamethoxazole 1000 µg/mL in methanol purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2023 Mar 15:253:114711. [Abstract]
Sulfamethoxazole (SMX) (1 μg/L; 4 weeks) significantly increased the ACE index, but significantly reduced the Simpson's index.
Sulfamethoxazole 1000 µg/mL in methanol purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2023 Mar 15:253:114711. [Abstract]
Sulfamethoxazole (SMX) (1 μg/L; 4 weeks) considerably increased the relative abundances of potential pathogens.
Sulfamethoxazole 1000 µg/mL in methanol purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2023 Mar 15:253:114711. [Abstract]
Sulfamethoxazole (SMX) (1 μg/L; 4 weeks). Heat map of some of representative genes related to immunity and drug metabolism in seahorse brood pouches.
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Antibiotics (Basel)
Revitalizing Trimethoprim/Sulfamethoxazole via Nanotechnology for Improved Pharmacokinetics and Antibacterial Efficacy. [Abstract]2026 Mar 10;15(3):283. PMID: 41892445 -
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Microorganisms
Isolation, Antimicrobial Susceptibility, and Genotypes of Three Pasteurellaeae Species Prevalent on Pig Farms in China Between 2021 and 2023. [Abstract]2025 Apr 18;13(4):938. PMID: 40284774 -
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Eur J Clin Microbiol Infect Dis
Evaluation of EUCAST rapid antimicrobial susceptibility testing (RAST) for carbapenemase-producing Klebsiella pneumoniae with focus on KPC variants. [Abstract]2025 Sep 29. PMID: 41023358 -
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Chemosphere
Mass-balance-model-based evaluation of sewage treatment plant contribution to residual pharmaceuticals in environmental waters. [Abstract]2019 Jun:225:378-387. PMID: 30884299
Purity & Documentation
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Data Sheet (275 KB)
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SDS (563 KB)
- English - EN (563 KB)
- Français - FR (563 KB)
- Deutsch - DE (563 KB)
- Norwegian - NO (563 KB)
- Español - ES (563 KB)
- Swedish - SV (563 KB)
- Italian - IT (563 KB)
- Korean - KR (563 KB)
- Portuguese - PT (563 KB)
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Handling Instructions (2659 KB)
References
[1]. Zambuzzi-Carvalho et al. Transcriptional profile of the human pathogenic fungus Paracoccidioides lutzii in response to sulfamethoxazole. Med Mycol. 2015;53(5):477-492. [Content Brief]
[2]. Choonara BF, et al. A menthol-based solid dispersion technique for enhanced solubility and dissolution of sulfamethoxazole from an oral tablet matrix. AAPS PharmSciTech. 2015 Aug;16(4):771-86. [Content Brief]
[3]. Ogata H, et al. Dose Dependent Effect of Sulfamethoxazole on Inhibiting KATP Channel of Mouse Pancreatic β Cell. Dose Response. 2023 Sep 28;21(3):15593258231203611. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)