Sulfamethoxazole (Synonyms: Ro 4-2130)

Name: Sulfamethoxazole
Brand: MedChemExpress
SKU: HY-B0322
Rating: 5.0 (24 reviews)

Cat. No.: HY-B0322 Purity: 99.70%: Data Sheet
COA

SDS
Handling Instructions
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Sulfamethoxazole (Ro 4-2130) is a sulfonamide antibiotic with a widespread antibacterial activity. Sulfamethoxazole inhibits bacterial folate metabolism by competing with 4-Aminobenzoic acid (HY-B1008) (PABA) to act on dihydropteroate synthetase and dihydropteroate reductase. Sulfamethoxazole can be used for the study of urinary tract infections (UTIs), prostatitis, and bronchitis.

For research use only. We do not sell to patients.

CAS No. : 723-46-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
25 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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Customer Review

Based on 24 publication(s) in Google Scholar

Other Forms of Sulfamethoxazole:

Sulfamethoxazole-d₄ In-stock
Sulfamethoxazole-¹³C₆ In-stock
Sulfamethoxazole sodium In-stock
Sulfamethoxazole 1000 µg/mL in methanol In-stock
Sulfamethoxazole (Standard) In-stock

24 Publications Citing Use of MCE Sulfamethoxazole

Bio/Physico-chemical Assay

: Sulfamethoxazole purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2023 Mar 15:253:114711. [Abstract]; Heat map of several key genes involved in immunity, metabolism, and circadian rhythm within the gut of juvenile and adult seahorses treated with Sulfamethoxazole (SMX) (1 μg/L; 4 weeks) was constructed using Tbtools software.

: Sulfamethoxazole purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2023 Mar 15:253:114711. [Abstract]; Sulfamethoxazole (SMX) (1 μg/L; 4 weeks) increased the relative abundance of Proteobacteria, whereas decreased the relative abundance of Actinobacteria and Acidobacteria.

: Sulfamethoxazole purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2023 Mar 15:253:114711. [Abstract]; Sulfamethoxazole (SMX) (1 μg/L; 4 weeks) significantly increased the ACE index, but significantly reduced the Simpson's index.

: Sulfamethoxazole purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2023 Mar 15:253:114711. [Abstract]; Sulfamethoxazole (SMX) (1 μg/L; 4 weeks) considerably increased the relative abundances of potential pathogens.

: Sulfamethoxazole purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2023 Mar 15:253:114711. [Abstract]; Sulfamethoxazole (SMX) (1 μg/L; 4 weeks). Heat map of some of representative genes related to immunity and drug metabolism in seahorse brood pouches.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Antibacterial

Cellular Effect

Cell Line	Type	Value	Description	References
A7R5	IC₅₀	333 μM Compound: 5a	Displacement of [125 I] ET-1 from rat aortic smooth muscle A7r5 cells transfected with Endothelin A receptor Displacement of [125 I] ET-1 from rat aortic smooth muscle A7r5 cells transfected with Endothelin A receptor	[PMID: 10969989]
A7R5	IC₅₀	333000 nM Compound: 31	Displacement of 125I-ET1 from ETA receptor in rat A7R5 cells Displacement of 125I-ET1 from ETA receptor in rat A7R5 cells	[PMID: 27321813]
HepG2	IC₅₀	2622 μM Compound: 1a, SMX	Cytotoxicity against human HepG2 cells after 24 hrs by MTS assay Cytotoxicity against human HepG2 cells after 24 hrs by MTS assay	[PMID: 22365879]
MT4	ED₅₀	> 79 μM Compound: A047	Effective dose of compound required to inhibit replication of human immunodeficiency virus type 1 in MT-4 cells Effective dose of compound required to inhibit replication of human immunodeficiency virus type 1 in MT-4 cells	[PMID: 15537350]

In Vitro

Sulfamethoxazole (0.01-1.28 mg/mL; 120 h) inhibits P. lutzii mitochondrial activity^[1].
Sulfamethoxazole (0.01 mg/mL; 1-2 h) acts in P. lutzii as a competitor for amino acid, nucleic acids and folate cofactor biosynthesis, disrupting mitochondrial functions^[1].
Sulfamethoxazole at different concentrations, by using the whole-cell patch clamp recording method, inhibits the K_ATP channel currents in mouse pancreatic β cells in a dose-dependent manner, and the IC₅₀ is 0.46 mM^[3].
Sulfamethoxazole at different concentrations, through the static incubation assay of islets, stimulates insulin secretion in mouse islets. It can stimulate insulin secretion at a low concentration (0.1 mM) under high-glucose (10 mM) conditions and only at a high concentration (10 mM) under low-glucose (2 mM) conditions^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	P. lutzii yeast cells
Concentration:	1.28 mg/mL, 0.64 mg/mL, 0.32 mg/mL, 0.16 mg/mL, 0.08 mg/mL, 0.04 mg/mL, 0.02 mg/mL, and 0.01 mg/mL
Incubation Time:	120 h
Result:	Produced a marked dose-dependent adverse effect on P. lutzii.

RT-PCR^[1]

Cell Line:	P. lutzii yeast cells
Concentration:	0.01 mg/mL
Incubation Time:	1 or 2 h
Result:	The up-regulated genes included those for HSP70, HSP60, and HSP30. The down-regulated genes included betaine aldehyde dehydrogenase (BADH), carnitine/acyl carnitine carrier (ACUH), guanine nucleotide binding protein α-1 subunit (GPA2), C6 transcription factor (CTF1B) and pleckstrin homology domain (PH).

Clinical Trial

Molecular Weight

253.28

Formula

C₁₀H₁₁N₃O₃S

CAS No.

723-46-6

Appearance

Solid

Color

White to off-white

SMILES

O=S(C1=CC=C(N)C=C1)(NC2=NOC(C)=C2)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (394.82 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.9482 mL	19.7410 mL	39.4820 mL
5 mM	0.7896 mL	3.9482 mL	7.8964 mL
10 mM	0.3948 mL	1.9741 mL	3.9482 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.70%

Select Batch:

Data Sheet (279 KB) SDS (419 KB)

COA (248 KB) LCMS (103 KB)

Handling Instructions (2659 KB)

References

[1]. Zambuzzi-Carvalho et al. Transcriptional profile of the human pathogenic fungus Paracoccidioides lutzii in response to sulfamethoxazole. Med Mycol. 2015;53(5):477-492. [Content Brief]

[2]. Choonara BF, et al. A menthol-based solid dispersion technique for enhanced solubility and dissolution of sulfamethoxazole from an oral tablet matrix. AAPS PharmSciTech. 2015 Aug;16(4):771-86. [Content Brief]

[3]. Ogata H, et al. Dose Dependent Effect of Sulfamethoxazole on Inhibiting KATP Channel of Mouse Pancreatic β Cell. Dose Response. 2023 Sep 28;21(3):15593258231203611. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.9482 mL	19.7410 mL	39.4820 mL	98.7050 mL
	5 mM	0.7896 mL	3.9482 mL	7.8964 mL	19.7410 mL
	10 mM	0.3948 mL	1.9741 mL	3.9482 mL	9.8705 mL
	15 mM	0.2632 mL	1.3161 mL	2.6321 mL	6.5803 mL
	20 mM	0.1974 mL	0.9870 mL	1.9741 mL	4.9352 mL
	25 mM	0.1579 mL	0.7896 mL	1.5793 mL	3.9482 mL
	30 mM	0.1316 mL	0.6580 mL	1.3161 mL	3.2902 mL
	40 mM	0.0987 mL	0.4935 mL	0.9870 mL	2.4676 mL
	50 mM	0.0790 mL	0.3948 mL	0.7896 mL	1.9741 mL
	60 mM	0.0658 mL	0.3290 mL	0.6580 mL	1.6451 mL
	80 mM	0.0494 mL	0.2468 mL	0.4935 mL	1.2338 mL
	100 mM	0.0395 mL	0.1974 mL	0.3948 mL	0.9870 mL

Sulfamethoxazole Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Sulfamethoxazole723-46-6Ro 4-2130AntibioticBacterialInhibitorinhibitorinhibit

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Sulfamethoxazole (Synonyms: Ro 4-2130)

Customer Review

Other Forms of Sulfamethoxazole:

Sulfamethoxazole Related Antibodies

24 Publications Citing Use of MCE Sulfamethoxazole

Featured Recommendations

•Related Screening Libraries:

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View All Antibiotic Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Sulfamethoxazole Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Sulfamethoxazole Related Classifications

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