Vitamin D3 octanoate
Based on 1 publication(s) in Google Scholar
Vitamin D3 octanoate is an octanoate ester of vitamin D3. Vitamin D3 (Cholecalciferol; HY-15398) is a naturally occuring form of vitamin D. Vitamin D3 induces cell differentiation and prevents proliferation of cancer cells.
For research use only. We do not sell to patients.
- Purity : 90.0%
- CAS No.: 927822-16-0
- Formula: C35H58O2
- Molecular Weight:510.83
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Storage:
-80°C, protect from light, stored under nitrogen
Publications Citing Use of MedChemExpress (MCE) Vitamin D3 octanoate
MoreAll Endogenous Metabolite Isoforms
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Biological Activity
Description
Chemical Information
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CAS No. 927822-16-0
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Appearance Oil
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Molecular Weight 510.83
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Formula C35H58O2
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Color Colorless to light yellow
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SMILES
CCCCCCCC(O[C@@H]1C/C(C(CC1)=C)=C/C=C2[C@]3([H])CC[C@H]([C@H](C)CCCC(C)C)[C@@]3(C)CCC/2)=O
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Structure Classification
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Initial Source
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Shipping
Shipping with dry ice.
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Storage
-80°C, protect from light, stored under nitrogen
Publications (1)
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Journal Impact Factor
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Most Recent
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Cell Metab
Sensing steroid hormone 17α-hydroxypregnenolone by GPR56 enables protection from ferroptosis-induced liver injury. [Abstract]2024 Nov 5;36(11):2402-2418.e10. PMID: 39389061
Solvent & Solubility
In Vitro:
DMSO : ≥ 100 mg/mL (195.76 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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iPSC cell differentiation
Induced pluripotent stem cells (iPSCs) are a type of cell that has similar properties to embryonic stem cells through somatic cell reprogramming.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (267 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. John M Ballard, et al. Degradation of vitamin D3 in a stressed formulation: the identification of esters of vitamin D3 formed by a transesterification with triglycerides. J Pharm Biomed Anal. 2007 Jan 4;43(1):142-50. [Content Brief]
[2]. Laura Bergadà, et al. Role of local bioactivation of vitamin D by CYP27A1 and CYP2R1 in the control of cell growth in normal endometrium and endometrial carcinoma. Lab Invest. 2014 Jun;94(6):608-22 [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9576 mL | 9.7880 mL | 19.5760 mL | 48.9400 mL |
| 5 mM | 0.3915 mL | 1.9576 mL | 3.9152 mL | 9.7880 mL | |
| 10 mM | 0.1958 mL | 0.9788 mL | 1.9576 mL | 4.8940 mL | |
| 15 mM | 0.1305 mL | 0.6525 mL | 1.3051 mL | 3.2627 mL | |
| 20 mM | 0.0979 mL | 0.4894 mL | 0.9788 mL | 2.4470 mL | |
| 25 mM | 0.0783 mL | 0.3915 mL | 0.7830 mL | 1.9576 mL | |
| 30 mM | 0.0653 mL | 0.3263 mL | 0.6525 mL | 1.6313 mL | |
| 40 mM | 0.0489 mL | 0.2447 mL | 0.4894 mL | 1.2235 mL | |
| 50 mM | 0.0392 mL | 0.1958 mL | 0.3915 mL | 0.9788 mL | |
| 60 mM | 0.0326 mL | 0.1631 mL | 0.3263 mL | 0.8157 mL | |
| 80 mM | 0.0245 mL | 0.1223 mL | 0.2447 mL | 0.6117 mL | |
| 100 mM | 0.0196 mL | 0.0979 mL | 0.1958 mL | 0.4894 mL |