Cat. No.: HY-P1425

(Z-LL)2 ketone

Customer Review

Based on 1 Customer Validation

(Z-LL)2 ketone is an inhibitor of ketone with an IC₅₀ value of 50 nM. (Z-LL)2 ketone inhibits processing of thep-Prlsignal peptide.

For research use only. We do not sell to patients.

Purity: 99.67%
CAS No.: 313664-40-3
Formula: C₄₃H₆₄N₆O₉
Molecular Weight:809.00

Storage:

Sealed storage, away from moisture.

Powder -80°C, 2 years , -20°C, 1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

(Z-LL)2 ketone Chemical Structure

CAS No.: 313664-40-3

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

(Z-LL)2 ketone is an inhibitor of ketone with an IC₅₀ value of 50 nM. (Z-LL)2 ketone inhibits processing of thep-Prlsignal peptide^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
HEK293	IC₅₀	>10 μM Compound: 41; (Z-LL)2	Inhibition of human gamma-secretase expressed in HEK293 cells using Notch1-VP16-Gal4 as substrate after 24 hrs by Bright-Glo luciferase reporter gene assay Inhibition of human gamma-secretase expressed in HEK293 cells using Notch1-VP16-Gal4 as substrate after 24 hrs by Bright-Glo luciferase reporter gene assay	[PMID: 29359565]
HEK293	IC₅₀	0.006 μM Compound: 41; (Z-LL)2	Inhibition of human SPPL2a expressed in HEK293 cells using GAL4-VP16 fusedTNFalpha (1 to 76)-NTF as substrate after 24 hrs by Bright-Glo luciferase reporter gene assay Inhibition of human SPPL2a expressed in HEK293 cells using GAL4-VP16 fusedTNFalpha (1 to 76)-NTF as substrate after 24 hrs by Bright-Glo luciferase reporter gene assay	[PMID: 29359565]
HEK293	IC₅₀	2 μM Compound: (Z-LL)2-ketone	Inhibition of SPP expressed in HEK293 cells in presence of Prl-PP peptide after 30 mins Inhibition of SPP expressed in HEK293 cells in presence of Prl-PP peptide after 30 mins	[PMID: 17932033]
HEK293	IC₅₀	5.3 nM Compound: (Z-LL)2-ketone	Displacement of [3H]L685458 from human SPP expressed in HEK293 cells Displacement of [3H]L685458 from human SPP expressed in HEK293 cells	[PMID: 17932033]
THP-1	IC₅₀	2060 nM Compound: (Z-LL)2-ketone	Displacement of [3H]IN973 from gamma-secretase in human THP1 cells Displacement of [3H]IN973 from gamma-secretase in human THP1 cells	[PMID: 17932033]
THP-1	IC₅₀	8.7 nM Compound: (Z-LL)2-ketone	Displacement of [3H]L685458 from gamma-secretase in human THP1 cells Displacement of [3H]L685458 from gamma-secretase in human THP1 cells	[PMID: 17932033]

Chemical Information

CAS No. 313664-40-3
Appearance Solid
Molecular Weight 809.00
Formula C₄₃H₆₄N₆O₉
Color White to off-white
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (123.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.2361 mL

6.1805 mL

12.3609 mL

5 mM

0.2472 mL

1.2361 mL

2.4722 mL

10 mM

0.1236 mL

0.6180 mL

1.2361 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (3.09 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (268 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. A Weihofen, et al. Release of signal peptide fragments into the cytosol requires cleavage in the transmembrane region by a protease activity that is specifically blocked by a novel cysteine protease inhibitor. J Biol Chem. 2000 Oct 6;275(40):30951-6. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.2361 mL	6.1805 mL	12.3609 mL	30.9023 mL
	5 mM	0.2472 mL	1.2361 mL	2.4722 mL	6.1805 mL
	10 mM	0.1236 mL	0.6180 mL	1.2361 mL	3.0902 mL
	15 mM	0.0824 mL	0.4120 mL	0.8241 mL	2.0602 mL
	20 mM	0.0618 mL	0.3090 mL	0.6180 mL	1.5451 mL
	25 mM	0.0494 mL	0.2472 mL	0.4944 mL	1.2361 mL
	30 mM	0.0412 mL	0.2060 mL	0.4120 mL	1.0301 mL
	40 mM	0.0309 mL	0.1545 mL	0.3090 mL	0.7726 mL
	50 mM	0.0247 mL	0.1236 mL	0.2472 mL	0.6180 mL
	60 mM	0.0206 mL	0.1030 mL	0.2060 mL	0.5150 mL
	80 mM	0.0155 mL	0.0773 mL	0.1545 mL	0.3863 mL
	100 mM	0.0124 mL	0.0618 mL	0.1236 mL	0.3090 mL

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(Z-LL)2 ketone

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

(Z-LL)2 ketone Related Classifications

HY-P1425 Related Classifications