β-Glucuronidase-IN-1
Based on 1 publication(s) in Google Scholar
β-Glucuronidase-IN-1 is a potent, selective, uncompetitive, and orally active E. coli bacterial β-glucuronidase inhibitor, exhibiting an IC50 and a Ki of 283 nM and 164 nM, respectively.
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- Purity : 98.21%
- CAS No.: 484006-66-8
- 화학식: C23H27N3O3S
- 분자량:425.54
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) β-Glucuronidase-IN-1
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Biological Activity
제품 설명
IC50 & Target
IC50: 283 nM (E. coli β-glucuronidase)Ki: 164 nM (E. coli β-glucuronidase)[1]
In Vitro
β-Glucuronidase-IN-1 (Inhibitor 1) (0.01-100 μM) inhibits E. coli β-glucuronidase activity as a dose-dependent manner and exhibits an IC50 and a Ki value with 283 nM and 164 nM, respectively[1].β-Glucuronidase-IN-1 (100 μM; 24-72 hours) maintains potent efficacy in living bacterial cells (EC50=17.7 nM), it does not affect bacterial cell growth under aerobic or anaerobic conditions or killing mammalian epithelial cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:CMT93, CaCO-2, HCT116 cells
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Concentration:100 μM
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Incubation Time:24 hours, 48 hours and 72 hours
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Result:Did not impact mammalian cell survival and any reduction in cell viability were attributed to the presence of DMSO.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Healthy 6- to 8-week-old Balb/cJ mice[1]
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Dosage:10 μg
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Administration:Oral gavage; twice per day; 11 days
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Result:Alleviated CPT-11-induced toxicity in mice.
Chemical Information
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CAS No. 484006-66-8
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Appearance Solid
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분자량 425.54
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화학식 C23H27N3O3S
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Color Light yellow to green yellow
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SMILES
S=C(NC1=CC=C(OCC)C=C1)N(CC2=CC3=C(NC2=O)C(C)=CC(C)=C3)CCO
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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Res Sq
2026 Feb 20:rs.3.rs-8856024. PMID: 41756457
용액&용해도
In Vitro:
DMSO : 100 mg/mL (235.00 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.87 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
순도&문서
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.3500 mL | 11.7498 mL | 23.4996 mL | 58.7489 mL |
| 5 mM | 0.4700 mL | 2.3500 mL | 4.6999 mL | 11.7498 mL | |
| 10 mM | 0.2350 mL | 1.1750 mL | 2.3500 mL | 5.8749 mL | |
| 15 mM | 0.1567 mL | 0.7833 mL | 1.5666 mL | 3.9166 mL | |
| 20 mM | 0.1175 mL | 0.5875 mL | 1.1750 mL | 2.9374 mL | |
| 25 mM | 0.0940 mL | 0.4700 mL | 0.9400 mL | 2.3500 mL | |
| 30 mM | 0.0783 mL | 0.3917 mL | 0.7833 mL | 1.9583 mL | |
| 40 mM | 0.0587 mL | 0.2937 mL | 0.5875 mL | 1.4687 mL | |
| 50 mM | 0.0470 mL | 0.2350 mL | 0.4700 mL | 1.1750 mL | |
| 60 mM | 0.0392 mL | 0.1958 mL | 0.3917 mL | 0.9791 mL | |
| 80 mM | 0.0294 mL | 0.1469 mL | 0.2937 mL | 0.7344 mL | |
| 100 mM | 0.0235 mL | 0.1175 mL | 0.2350 mL | 0.5875 mL |
β-Glucuronidase-IN-1 Related Classifications
HY-103081 Related Classifications
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