1. GPCR/G Protein
  2. Prostaglandin Receptor
  3. 16,16-Dimethyl prostaglandin E2

16,16-Dimethyl prostaglandin E2  (Synonyms: 16,16-dimethyl PGE2)

Cat. No.: HY-106420 Purity: ≥98.0%
COA Handling Instructions

16,16-Dimethyl prostaglandin E2 (16,16-dimethyl PGE2) is an orally active vertebrate Hematopoietic stem cells (HSCs) homeostasis critical regulator. 16,16-Dimethyl prostaglandin E2 can act through EP2/EP4 and has an interaction with the Wnt pathway.

For research use only. We do not sell to patients.

16,16-Dimethyl prostaglandin E2 Chemical Structure

16,16-Dimethyl prostaglandin E2 Chemical Structure

CAS No. : 39746-25-3

Size Price Stock Quantity
Solvent
5 mg (26.28 mM * 500 μL in Methyl acetate) USD 320 In-stock
Solvent
10 mg (26.28 mM * 1 mL in Methyl acetate) USD 510 In-stock
Solvent
25 mg (26.28 mM * 2.5 mL in Methyl acetate) USD 1050 In-stock
Solvent
50 mg (26.28 mM * 5 mL in Methyl acetate) USD 1680 In-stock
Solvent
100 mg (26.28 mM * 10 mL in Methyl acetate) USD 2650 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

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  • Biological Activity

  • Purity & Documentation

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Description

16,16-Dimethyl prostaglandin E2 (16,16-dimethyl PGE2) is an orally active vertebrate Hematopoietic stem cells (HSCs) homeostasis critical regulator. 16,16-Dimethyl prostaglandin E2 can act through EP2/EP4 and has an interaction with the Wnt pathway[1][2].

In Vivo

16,16-Dimethyl prostaglandin E2 (16,16-dimethyl PGE2; 10 µg/kg; i.p.; every 12 hours; for 8 days) rescues the exacerbated DSS colitis phenotype of Tpl2IMF-KO mice and restores epithelial proliferation and structure[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Tpl2IMF-KO mice[2]
Dosage: 10 µg/kg
Administration: IP; every 12 hours; for 8 days
Result: Rescued the exacerbated DSS colitis phenotype and restores epithelial proliferation and structure.
Molecular Weight

380.52

Formula

C22H36O5

CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

CCCCC(C)([C@@H](/C=C/[C@@H]1[C@@H](C/C=C\CCCC(O)=O)C(C[C@H]1O)=O)O)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Solution, -20°C, 2 years

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (262.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.57 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.57 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: ≥98.0%

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6280 mL 13.1399 mL 26.2798 mL 65.6996 mL
5 mM 0.5256 mL 2.6280 mL 5.2560 mL 13.1399 mL
10 mM 0.2628 mL 1.3140 mL 2.6280 mL 6.5700 mL
15 mM 0.1752 mL 0.8760 mL 1.7520 mL 4.3800 mL
20 mM 0.1314 mL 0.6570 mL 1.3140 mL 3.2850 mL
25 mM 0.1051 mL 0.5256 mL 1.0512 mL 2.6280 mL
30 mM 0.0876 mL 0.4380 mL 0.8760 mL 2.1900 mL
40 mM 0.0657 mL 0.3285 mL 0.6570 mL 1.6425 mL
50 mM 0.0526 mL 0.2628 mL 0.5256 mL 1.3140 mL
60 mM 0.0438 mL 0.2190 mL 0.4380 mL 1.0950 mL
80 mM 0.0328 mL 0.1642 mL 0.3285 mL 0.8212 mL
100 mM 0.0263 mL 0.1314 mL 0.2628 mL 0.6570 mL
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16,16-Dimethyl prostaglandin E2 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
16,16-Dimethyl prostaglandin E2
Cat. No.:
HY-106420
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