2′,3′-Dihydroxypuberulin
2′,3′-Dihydroxypuberulin is a naturally derived inhibitor of the FcεRI β-chain with FcεRI expression inhibitory activity in mast cells. 2′,3′-Dihydroxypuberulin suppresses the mRNA level of the FcεRI β-chain, blocks the assembly of the FcεRI tetramer, and reduces the cell surface expression level of FcεRI. 2′,3′-Dihydroxypuberulin can be used in studies related to type I allergic diseases.
For research use only. We do not sell to patients.
- CAS No.: 79147-77-6
- Formula: C16H20O7
- Molecular Weight:324.33
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
2′,3′-Dihydroxypuberulin (compound 1) (30 μg/mL; 72 h) inhibits FcεRI surface expression on HMC-1 human mast cells by 49.0%, and its absolute configuration does not affect this activity[2].
2′,3′-Dihydroxypuberulin (50-100 μM; 36 h) significantly down-regulates FcεRI β-chain mRNA expression without altering α-chain or γ-chain mRNA levels in HMC-1 human mast cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:human mast cell line HMC-1
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Concentration:50, 100 μM
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Incubation Time:36 h
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Result:Suppressed FcεRI β-chain mRNA expression to ~85% of vehicle control levels, while α-chain and γ-chain mRNA levels remained near 95-98% of vehicle control (100 μM).
Suppressed FcεRI β-chain mRNA expression to ~60% of vehicle control, while α-chain and γ-chain mRNA levels remained near 90-95% of vehicle control (50 μM).
Chemical Information
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CAS No. 79147-77-6
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Molecular Weight 324.33
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Formula C16H20O7
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SMILES
COC1=C2C(C=CC(O2)=O)=CC(OC)=C1OCC(O)C(C)(O)C
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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RNA extraction experimental
By lysing cells, releasing RNA, and removing impurities such as proteins and DNA, high-purity RNA products are finally obtained. The commonly used traditional method is the guanidine isothiocyanate/phenol/chloroform method (Trizol), which is suitable for a variety of animal materials including animal tissues, microorganisms, cultured cells, etc., and most plant materials.
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Real Time qPCR (Q-PCR)
Real-time quantitative PCR (qPCR) quantifies an amplifiable nucleic-acid target by monitoring fluorescence during PCR cycling rather than measuring product only after amplification. The increase in fluorescence tracks accumulation of PCR product, and the quantification cycle (Cq; historically also Ct/CP) is related to the initial amount of target: samples containing more starting target generally reach the defined fluorescence threshold in fewer cycles.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
2′,3′-Dihydroxypuberulin Related Classifications
HY-N19733 Related Classifications
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