2’-Deoxy-2’-fluoro-b-D-arabinocytidine
Based on 1 Customer Validation
2’-Deoxy-2’-fluoro-b-D-arabinocytidine is a purine nucleoside analog. Purine nucleoside analogs have broad antitumor activity targeting indolent lymphoid malignancies. Anticancer mechanisms in this process rely on inhibition of DNA synthesis, induction of apoptosis, etc.
For research use only. We do not sell to patients.
- Purity: 99.92%
- CAS No.: 56632-83-8
- Formula: C9H12FN3O4
- Molecular Weight:245.21
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CCRF-CEM | IC50 |
>100 μM
Compound: 27
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The growth inhibition activity for the compound was evaluated in CEM cells.
The growth inhibition activity for the compound was evaluated in CEM cells.
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[PMID: 8709113] |
| HepG2 2.2.15 | EC50 |
1.4 μM
Compound: 27
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Anti hepatitis-B virus activity was evaluated in 2.2.15 cells.
Anti hepatitis-B virus activity was evaluated in 2.2.15 cells.
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[PMID: 8709113] |
| HepG2 2.2.15 | IC50 |
>200 μM
Compound: 27
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The growth inhibition activity for the compound was evaluated in 2.2.15 cells.
The growth inhibition activity for the compound was evaluated in 2.2.15 cells.
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[PMID: 8709113] |
| Huh-7 | CC50 |
>100 μM
Compound: 2'-FdCyd
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Cytotoxic concentration against HCV replication RNA levels in Huh7 cells determined by HCV replicon assay
Cytotoxic concentration against HCV replication RNA levels in Huh7 cells determined by HCV replicon assay
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[PMID: 16107149] |
| Huh-7 | CC50 |
>50 μM
Compound: 10
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Cytotoxicity against human HuH7 cells by MTS assay
Cytotoxicity against human HuH7 cells by MTS assay
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[PMID: 20580554] |
Chemical Information
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CAS No. 56632-83-8
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Appearance Solid
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Molecular Weight 245.21
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Formula C9H12FN3O4
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Color White to off-white
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SMILES
O[C@H]1[C@H](F)[C@H](N2C=CC(N)=NC2=O)O[C@@H]1CO
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Synonyms
4-amino-1-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl)pyrimidin-2(1H)-one
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (407.81 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (10.20 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (10.20 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.0781 mL | 20.3907 mL | 40.7814 mL | 101.9534 mL |
| 5 mM | 0.8156 mL | 4.0781 mL | 8.1563 mL | 20.3907 mL | |
| 10 mM | 0.4078 mL | 2.0391 mL | 4.0781 mL | 10.1953 mL | |
| 15 mM | 0.2719 mL | 1.3594 mL | 2.7188 mL | 6.7969 mL | |
| 20 mM | 0.2039 mL | 1.0195 mL | 2.0391 mL | 5.0977 mL | |
| 25 mM | 0.1631 mL | 0.8156 mL | 1.6313 mL | 4.0781 mL | |
| 30 mM | 0.1359 mL | 0.6797 mL | 1.3594 mL | 3.3984 mL | |
| 40 mM | 0.1020 mL | 0.5098 mL | 1.0195 mL | 2.5488 mL | |
| 50 mM | 0.0816 mL | 0.4078 mL | 0.8156 mL | 2.0391 mL | |
| 60 mM | 0.0680 mL | 0.3398 mL | 0.6797 mL | 1.6992 mL | |
| 80 mM | 0.0510 mL | 0.2549 mL | 0.5098 mL | 1.2744 mL | |
| 100 mM | 0.0408 mL | 0.2039 mL | 0.4078 mL | 1.0195 mL |
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