2-Hydroxy-5-nitrobenzoic acid, 99%
Based on 1 Customer Validation
2-Hydroxy-5-nitrobenzoic acid, 99% is used as an HPLC analytical standard, pH indicator, and heavy metal detection agent, and is also used in the study of phenolic compounds oxidation.
For research use only. We do not sell to patients.
- Purity: 96.80%
- CAS No.: 96-97-9
- Formula: C7H5NO5
- Molecular Weight:183.12
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Storage:
Store at room temperature 3 years.
In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | IC50 |
5.3 mM
Compound: 5
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Cytotoxicity against HEK293 cells harboring pendrin P123S mutant after 72 hrs by MTT assay
Cytotoxicity against HEK293 cells harboring pendrin P123S mutant after 72 hrs by MTT assay
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[PMID: 28341401] |
Chemical Information
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CAS No. 96-97-9
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Appearance Solid
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Molecular Weight 183.12
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Formula C7H5NO5
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Color Light yellow to brown
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SMILES
O=C(C1=CC([N+]([O-])=O)=CC=C1O)O
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Synonyms
Anilotic acid, 99%
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Store at room temperature 3 years
In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : 100 mg/mL (546.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (13.65 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (13.65 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (245 KB)
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SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
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Handling Instructions (2659 KB)
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.4609 mL | 27.3045 mL | 54.6090 mL | 136.5225 mL |
| 5 mM | 1.0922 mL | 5.4609 mL | 10.9218 mL | 27.3045 mL | |
| 10 mM | 0.5461 mL | 2.7305 mL | 5.4609 mL | 13.6523 mL | |
| 15 mM | 0.3641 mL | 1.8203 mL | 3.6406 mL | 9.1015 mL | |
| 20 mM | 0.2730 mL | 1.3652 mL | 2.7305 mL | 6.8261 mL | |
| 25 mM | 0.2184 mL | 1.0922 mL | 2.1844 mL | 5.4609 mL | |
| 30 mM | 0.1820 mL | 0.9102 mL | 1.8203 mL | 4.5508 mL | |
| 40 mM | 0.1365 mL | 0.6826 mL | 1.3652 mL | 3.4131 mL | |
| 50 mM | 0.1092 mL | 0.5461 mL | 1.0922 mL | 2.7305 mL | |
| 60 mM | 0.0910 mL | 0.4551 mL | 0.9102 mL | 2.2754 mL | |
| 80 mM | 0.0683 mL | 0.3413 mL | 0.6826 mL | 1.7065 mL | |
| 100 mM | 0.0546 mL | 0.2730 mL | 0.5461 mL | 1.3652 mL |
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