3-Hydroxypicolinic acid
Based on 1 Customer Validation
3-Hydroxypicolinic acid is a heterocyclic carboxylic acid ligand and cytotoxin, with a MIC90 of >25 μg/mL against Mycobacterium tuberculosis H37Rv. 3-Hydroxypicolinic acid inhibits the growth of cancer cells and normal fibroblasts. 3-Hydroxypicolinic acid is applicable to research related to chronic myeloid leukemia, human lung adenocarcinoma, and tuberculosis.
For research use only. We do not sell to patients.
- Purity: 99.92%
- CAS No.: 874-24-8
- Formula: C6H5NO3
- Molecular Weight:139.11
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Human Endogenous Metabolite |
3-Hydroxypicolinic acid (72 h) inhibits K562 chronic myelogenous leukemia cell proliferation with an IC50 of 130.0 μM following 72 h of incubation[1].
3-Hydroxypicolinic acid (24-72 h) inhibits MRC-5 normal pulmonary fibroblast cell proliferation with IC50 values of 2767.6 μM (72 h incubation) and 2228.4 μM (24 h incubation)[1].
3-Hydroxypicolinic acid (24 h) inhibits A549 human lung adenocarcinoma epithelial cell proliferation with an IC50 of 2264.4 μM following 24 h of incubation[1].
3-Hydroxypicolinic acid (7 days) does not inhibit Mycobacterium tuberculosis H37Rv growth at concentrations up to 179.7 μM following 7 days of incubation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:K562 chronic myelogenous leukemia cells
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Concentration:130.0 μM
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Incubation Time:72 h
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Result:Inhibited 50% of K562 cell proliferation at a concentration of 130.0 μM after 72 h of incubation.
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Cell Line:MRC-5 normal pulmonary fibroblast cells
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Concentration:2767.6 μM (72 h incubation); 2228.4 μM (24 h incubation)
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Incubation Time:72 h, 24 h
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Result:Inhibited 50% of MRC-5 cell proliferation at a concentration of 2767.6 μM after 72 h of incubation.
Inhibited 50% of MRC-5 cell proliferation at a concentration of 2228.4 μM after 24 h of incubation.
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Cell Line:A549 human lung adenocarcinoma epithelial cells
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Concentration:2264.4 μM
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Incubation Time:24 h
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Result:Inhibited 50% of A549 cell proliferation at a concentration of 2264.4 μM after 24 h of incubation.
Chemical Information
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CAS No. 874-24-8
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Appearance Solid
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Molecular Weight 139.11
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Formula C6H5NO3
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Color White to light yellow
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SMILES
O=C(C1=NC=CC=C1O)O
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Synonyms
3-hydroxypyridine-2-carboxylic acid
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 50 mg/mL (359.43 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (8.99 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (8.99 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (306 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 7.1886 mL | 35.9428 mL | 71.8856 mL | 179.7139 mL |
| 5 mM | 1.4377 mL | 7.1886 mL | 14.3771 mL | 35.9428 mL | |
| 10 mM | 0.7189 mL | 3.5943 mL | 7.1886 mL | 17.9714 mL | |
| 15 mM | 0.4792 mL | 2.3962 mL | 4.7924 mL | 11.9809 mL | |
| 20 mM | 0.3594 mL | 1.7971 mL | 3.5943 mL | 8.9857 mL | |
| 25 mM | 0.2875 mL | 1.4377 mL | 2.8754 mL | 7.1886 mL | |
| 30 mM | 0.2396 mL | 1.1981 mL | 2.3962 mL | 5.9905 mL | |
| 40 mM | 0.1797 mL | 0.8986 mL | 1.7971 mL | 4.4928 mL | |
| 50 mM | 0.1438 mL | 0.7189 mL | 1.4377 mL | 3.5943 mL | |
| 60 mM | 0.1198 mL | 0.5990 mL | 1.1981 mL | 2.9952 mL | |
| 80 mM | 0.0899 mL | 0.4493 mL | 0.8986 mL | 2.2464 mL | |
| 100 mM | 0.0719 mL | 0.3594 mL | 0.7189 mL | 1.7971 mL |
3-Hydroxypicolinic acid Related Classifications
HY-Y0030 Related Classifications
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- 3-Hydroxypicolinic acid
- 874-24-8
- 3-hydroxypyridine-2-carboxylic acid
- Biochemical Assay Reagents
- K562 chronic myelogenous leukemia cell
- A549 human lung adenocarcinoma epithelial cell
- normal fibroblasts
- human lung adenocarcinoma
- chronic myelogenous leukemia
- tuberculosis
- cancer cells
- MRC-5 normal pulmonary fibroblast cell
- Mycobacterium tuberculosis H37Rv
- oligodeoxynucleotides
- Inhibitor
- inhibitor
- inhibit