3′-Deoxy-3′-fluoroguanosine
3′-Deoxy-3′-fluoroguanosine exhibits antiviral activity against tick-borne encephalitis virus (TBEV), through interaction with NS5B RdRp of HCV, resulting in suppression of viral RNA synthesis by disruption of further extension of the replicating viral RNA.
For research use only. We do not sell to patients.
- CAS No.: 123402-21-1
- Formula: C10H12FN5O4
- Molecular Weight:285.23
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| L1210 | IC50 |
5 μg/mL
Compound: 27
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Concentration required to reduce the viability of Murine leukemia L1210 cells
Concentration required to reduce the viability of Murine leukemia L1210 cells
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[PMID: 2066992] |
| Macrophage | EC50 |
>64 μM
Compound: 16
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Cytotoxicity against mouse primary macrophage cells assessed as reduction in cell viability
Cytotoxicity against mouse primary macrophage cells assessed as reduction in cell viability
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[PMID: 33667845] |
| MOLT-4F | IC50 |
75 μg/mL
Compound: 27
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Concentration required to reduce the viability of Human T-lymphoblast Molt/4F cells
Concentration required to reduce the viability of Human T-lymphoblast Molt/4F cells
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[PMID: 2066992] |
| MRC5 | EC50 |
42 μM
Compound: 16
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Cytotoxicity against human MRC5 cells assessed as reduction in cell viability after 3 days by resazurin dye based fluorimetric analysis
Cytotoxicity against human MRC5 cells assessed as reduction in cell viability after 3 days by resazurin dye based fluorimetric analysis
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[PMID: 33667845] |
| MT4 | IC50 |
>100 μg/mL
Compound: 27
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Concentration required to reduce the viability of Human T-lymphoblast MT-4 cells
Concentration required to reduce the viability of Human T-lymphoblast MT-4 cells
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[PMID: 2066992] |
| Raji | IC50 |
29 μg/mL
Compound: 27
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Concentration required to reduce the viability of Human B-lymphoblast Raji cells
Concentration required to reduce the viability of Human B-lymphoblast Raji cells
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[PMID: 2066992] |
Chemical Information
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CAS No. 123402-21-1
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Appearance Solid
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Molecular Weight 285.23
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Formula C10H12FN5O4
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SMILES
NC(NC1=O)=NC2=C1N=CN2[C@@H]3O[C@H](CO)[C@@H](F)[C@H]3O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 20 mg/mL (70.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2 mg/mL (7.01 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2 mg/mL (7.01 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (555 KB)
- English - EN (555 KB)
- Français - FR (555 KB)
- Deutsch - DE (555 KB)
- Norwegian - NO (555 KB)
- Español - ES (555 KB)
- Swedish - SV (555 KB)
- Italian - IT (555 KB)
- Korean - KR (555 KB)
- Portuguese - PT (555 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.5059 mL | 17.5297 mL | 35.0594 mL | 87.6486 mL |
| 5 mM | 0.7012 mL | 3.5059 mL | 7.0119 mL | 17.5297 mL | |
| 10 mM | 0.3506 mL | 1.7530 mL | 3.5059 mL | 8.7649 mL | |
| 15 mM | 0.2337 mL | 1.1686 mL | 2.3373 mL | 5.8432 mL | |
| 20 mM | 0.1753 mL | 0.8765 mL | 1.7530 mL | 4.3824 mL | |
| 25 mM | 0.1402 mL | 0.7012 mL | 1.4024 mL | 3.5059 mL | |
| 30 mM | 0.1169 mL | 0.5843 mL | 1.1686 mL | 2.9216 mL | |
| 40 mM | 0.0876 mL | 0.4382 mL | 0.8765 mL | 2.1912 mL | |
| 50 mM | 0.0701 mL | 0.3506 mL | 0.7012 mL | 1.7530 mL | |
| 60 mM | 0.0584 mL | 0.2922 mL | 0.5843 mL | 1.4608 mL |
3′-Deoxy-3′-fluoroguanosine Related Classifications
HY-W128400 Related Classifications
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