2. Anti-infection
  3. RSV SARS-CoV HCV
  4. EIDD-2749

EIDD-2749  (Synonyms: 4'-FlU; 4'-Fluorouridine)

Cat. No.: HY-146246 Purity: 99.85%
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EIDD-2749 (4'-Fluorouridine) is an orally active RdRp inhibitor. EIDD-2749 effectively blocks the replication of RSV and SARS-CoV-2. EIDD-2749 also exhibits activity against HCV and lymphocytic choriomeningitis virus (LCMV). EIDD-2749 is a promising oral therapeutic candidate for COVID-19 and is also suitable for research on other RNA viruses.

For research use only. We do not sell to patients.

EIDD-2749 Chemical Structure

EIDD-2749 Chemical Structure

CAS No. : 1613589-24-4

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 374 In-stock
Solution
10 mM * 1 mL in DMSO USD 374 In-stock
Solid
5 mg USD 340 In-stock
10 mg USD 550 In-stock
25 mg USD 1100 In-stock
50 mg USD 1800 In-stock
100 mg USD 2900 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

EIDD-2749 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

  • References

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Description

EIDD-2749 (4'-Fluorouridine) is an orally active RdRp inhibitor. EIDD-2749 effectively blocks the replication of RSV and SARS-CoV-2. EIDD-2749 also exhibits activity against HCV and lymphocytic choriomeningitis virus (LCMV). EIDD-2749 is a promising oral therapeutic candidate for COVID-19 and is also suitable for research on other RNA viruses[1][2][3].

IC50 & Target

RdRp, RSV,SARS-CoV-2, HCV, COVID-19, LCMV[1][2][3].
In Vitro

EIDD-2749 induces a delayed stalling of phosphodiester bond formation by RSV and SARS-CoV-2 RdRP[1].
EIDD-2749 is rapidly anabolizes, metabolically stable, and potently antiviral in disease-relevant well-differentiated HAE cultures[1].
EIDD-2749 shows a ≥17-fold increase in anti-RSV potency relative to that on HEp-2 cells; however, the low cytotoxicity levels remains unchanged (CC50 169 mM), resulting in a high SI (SI = EC50/CC50) of ≥1877[1].
EIDD-2749 inhibits SARS-CoV-2 with an EC50 value of 0.2-0.6 M[2].
EIDD-2749 has an EC50 of 1.86 μM in theVero E6 cell line, cytotoxicity with a CC50 of 380 μM, and stability in human plasma[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

EIDD-2749 Related Antibodies
In Vivo

EIDD-2749 (0.2, 1, 5 mg/kg; p.o.; single daily for 4 days) shows good orally efficacious in RSV infection mice model in a dose-dependent manner[1].
EIDD-2749 shows high efficacious to SARS-CoV-2 infection and is effective with a single daily dose versus molnupiravir administered twice daily in vivo[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Balb/cJ mice (RSV infection model)[1].
Dosage: 0.2, 1, 5 mg/kg
Administration: Oral administration; single daily for 4 days
Result: Resulted in a statistically significant reduction in lung virus load.
Molecular Weight

262.19

Formula

C9H11FN2O6
CAS No.
Appearance

Solid

Color

Off-white to yellow

SMILES

O=C1NC(N([C@@H]2O[C@@](CO)(F)[C@@H](O)[C@H]2O)C=C1)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 62.5 mg/mL (238.38 mM; Need ultrasonic)

DMSO : 25 mg/mL (95.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.8140 mL 19.0701 mL 38.1403 mL
5 mM 0.7628 mL 3.8140 mL 7.6281 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 25 mg/mL (95.35 mM); Clear solution

    This protocol yields a clear solution of ≥ 25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (250.0 mg/mL) to 900 μL Corn oil, and mix evenly.

  • Protocol 2

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (9.54 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 3.8140 mL 19.0701 mL 38.1403 mL 95.3507 mL
5 mM 0.7628 mL 3.8140 mL 7.6281 mL 19.0701 mL
10 mM 0.3814 mL 1.9070 mL 3.8140 mL 9.5351 mL
15 mM 0.2543 mL 1.2713 mL 2.5427 mL 6.3567 mL
20 mM 0.1907 mL 0.9535 mL 1.9070 mL 4.7675 mL
25 mM 0.1526 mL 0.7628 mL 1.5256 mL 3.8140 mL
30 mM 0.1271 mL 0.6357 mL 1.2713 mL 3.1784 mL
40 mM 0.0954 mL 0.4768 mL 0.9535 mL 2.3838 mL
50 mM 0.0763 mL 0.3814 mL 0.7628 mL 1.9070 mL
60 mM 0.0636 mL 0.3178 mL 0.6357 mL 1.5892 mL
80 mM 0.0477 mL 0.2384 mL 0.4768 mL 1.1919 mL
H2O 100 mM 0.0381 mL 0.1907 mL 0.3814 mL 0.9535 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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EIDD-2749 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

EIDD-27491613589-24-44'-FlU 4'-FluorouridineEIDD2749EIDD 2749RSVSARS-CoVHCVRespiratory syncytial virusSARS coronavirusHepatitis C virusSARSCoV-2HAELCMVCOVID-19Vero E6 cellInhibitorinhibitorinhibit

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