3-epi-18β-Glycyrrhetinic acid
3-epi-18β-Glycyrrhetinic acid is an inhibitor of 11β-HSD1 and 11β-HSD2, with an IC50 of 0.17 μM against 11β-HSD2. 3-epi-18β-Glycyrrhetinic acid acts as a weak substrate for sulfotransferase 2A1. It serves as a biomarker for the toxicity of licorice-related pseudoaldosteronism. 3-epi-18β-Glycyrrhetinic acid is applicable to studies related to pseudoaldosteronism.
For research use only. We do not sell to patients.
- CAS No.: 90365-60-9
- Formula: C30H46O4
- Molecular Weight:470.69
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
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11β-HSD1 |
11β-HSD2 0.17 μM (IC50) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| 518A2 | IC50 |
30 μmol
Compound: 3
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Cytotoxicity against human 518A2 cells after 96 hrs by SRB assay
Cytotoxicity against human 518A2 cells after 96 hrs by SRB assay
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[PMID: 21959232] |
| A-431 | IC50 |
30 μmol
Compound: 3
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Cytotoxicity against human A431 cells after 96 hrs by SRB assay
Cytotoxicity against human A431 cells after 96 hrs by SRB assay
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[PMID: 21959232] |
| A549 | IC50 |
30 μmol
Compound: 3
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Cytotoxicity against human A549 cells after 96 hrs by SRB assay
Cytotoxicity against human A549 cells after 96 hrs by SRB assay
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[PMID: 21959232] |
| DLD-1 | IC50 |
30 μmol
Compound: 3
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Cytotoxicity against human DLD1 cells after 96 hrs by SRB assay
Cytotoxicity against human DLD1 cells after 96 hrs by SRB assay
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[PMID: 21959232] |
| HCT-116 | IC50 |
30 μmol
Compound: 3
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Cytotoxicity against human HCT116 cells after 96 hrs by SRB assay
Cytotoxicity against human HCT116 cells after 96 hrs by SRB assay
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[PMID: 21959232] |
| HCT-8 | IC50 |
30 μmol
Compound: 3
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Cytotoxicity against human HCT8 cells after 96 hrs by SRB assay
Cytotoxicity against human HCT8 cells after 96 hrs by SRB assay
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[PMID: 21959232] |
| HT-29 | IC50 |
30 μmol
Compound: 3
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Cytotoxicity against human HT-29 cells after 96 hrs by SRB assay
Cytotoxicity against human HT-29 cells after 96 hrs by SRB assay
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[PMID: 21959232] |
| MCF7 | IC50 |
30 μmol
Compound: 3
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Cytotoxicity against human MCF7 cells after 96 hrs by SRB assay
Cytotoxicity against human MCF7 cells after 96 hrs by SRB assay
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[PMID: 21959232] |
| SW-1736 | IC50 |
30 μmol
Compound: 3
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Cytotoxicity against human SW1736 cells after 96 hrs by SRB assay
Cytotoxicity against human SW1736 cells after 96 hrs by SRB assay
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[PMID: 21959232] |
In Vitro
3-epi-18β-Glycyrrhetinic acid (Compound 12) (1 μM; 10 min) potently inhibits recombinant h11β-HSD1 and h11β-HSD2, with inhibition rates of 97.4% and 97.0% at the concentration of 1 μM, respectively[1].
3-epi-18β-Glycyrrhetinic acid (30 min) potently inhibits 11β-HSD2 in vitro, with a mean IC50 of 0.17 μM[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 90365-60-9
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Molecular Weight 470.69
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Formula C30H46O4
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SMILES
C[C@]12[C@@]([C@]3(C)[C@@](CC1)(C(C)(C)[C@H](O)CC3)[H])(C(=O)C=C4[C@@]2(C)CC[C@]5(C)[C@]4(C[C@](C(O)=O)(C)CC5)[H])[H]
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
References
[1]. Beseda I, et al. Synthesis of glycyrrhetinic acid derivatives for the treatment of metabolic diseases. Bioorganic & medicinal chemistry. 2010 Jan 01;18(1):433-54. [Content Brief]
[2]. Sakoda R, et al. Stereoisomerism at the 3-position of glycyrrhetinic acid affects pseudoaldosteronism-related toxicokinetics. Drug metabolism and disposition: the biological fate of chemicals. 2025 Nov;53(11):100180. [Content Brief]
[3]. Sakoda R, et al. 3--18-Glycyrrhetinic Acid or Its Glucuronide, the Metabolites of Glycyrrhizinic Acid with Individual Differences, Correlated with Diagnostic Marker for Licorice-Induced Pseudoaldosteronism in Humans. Drug metabolism and disposition: the biological fate of chemicals. 2024 Nov 15;52(12):1407-1416. [Content Brief]
[4]. Yoshino T, et al. The Role of 3-epi-18β-Glycyrrhetinic Acid and Hepatic Function in Licorice-Induced Pseudohyperaldosteronism. Kidney Int Rep. 2026 Jan 9;11(3):103771. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
3-epi-18β-Glycyrrhetinic acid Related Classifications
HY-N0375A Related Classifications
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